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Literature summary extracted from

  • Oshima, M.; Yamamoto, T.; Soda, K.
    Further characterization of glutaminase isoenzymes from Pseudomonas aeruginosa (1976), Agric. Biol. Chem., 40, 2251-2256.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 0.71
-
 L-Theanine glutaminase B Pseudomonas aeruginosa
3.5.1.2 0.92
-
 D-theanine glutaminase B Pseudomonas aeruginosa
3.5.1.2 2.1
-
 L-gamma-Gln-methyl ester glutaminase B Pseudomonas aeruginosa
3.5.1.2 6.7
-
 D-gamma-Gln-methylester glutaminase B Pseudomonas aeruginosa
3.5.1.2 200
-
 L-gamma-glutamyl-L-Ala glutaminase B Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 35000
-
 4 * 35000, glutaminase A, SDS-PAGE Pseudomonas aeruginosa
3.5.1.2 67000
-
 4 * 67000, glutaminase B, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-Asn + H2O glutaminase A Pseudomonas aeruginosa D-Asp + NH3
-
 ?
3.5.1.2 D-Asn + hydroxylamine
-
 Pseudomonas aeruginosa D-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
-
 ?
3.5.1.2 D-gamma-glutamyl-ethylester + H2O
-
 Pseudomonas aeruginosa D-Glu + ethanol
-
 ?
3.5.1.2 D-gamma-glutamyl-ethylester + hydroxylamine
-
 Pseudomonas aeruginosa D-gamma-glutamyl hydroxamate + ethanol
-
 ?
3.5.1.2 D-gamma-glutamyl-hydrazide + H2O
-
 Pseudomonas aeruginosa D-Glu + hydroxyhydrazine
-
 ?
3.5.1.2 D-gamma-glutamyl-hydrazide + hydroxylamine
-
 Pseudomonas aeruginosa D-gamma-glutamyl hydroxamate + hydroxyhydrazine
-
 ?
3.5.1.2 D-gamma-glutamyl-methylester + H2O
-
 Pseudomonas aeruginosa D-Glu + methanol
-
 ?
3.5.1.2 D-gamma-glutamyl-methylester + hydroxylamine
-
 Pseudomonas aeruginosa L-gamma-glutamyl hydroxamate + methanol
-
 ?
3.5.1.2 D-Gln + H2O glutaminase A Pseudomonas aeruginosa D-Glu + NH3
-
 ?
3.5.1.2 D-Gln + hydroxylamine glutaminase A and B Pseudomonas aeruginosa D-gamma-glutamyl hydroxamate + NH3
-
 ?
3.5.1.2 D-theanine + H2O
-
 Pseudomonas aeruginosa D-glutamate + ethylamine
-
 ?
3.5.1.2 Gln + H2O
-
 Pseudomonas aeruginosa Glu + NH3
-
 ?
3.5.1.2 L-Asn + H2O glutaminase A Pseudomonas aeruginosa L-Asp + NH3
-
 ?
3.5.1.2 L-Asn + hydroxylamine
-
 Pseudomonas aeruginosa L-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
-
 ?
3.5.1.2 L-gamma-Gln-methyl ester + H2O
-
 Pseudomonas aeruginosa L-Gln + methanol
-
 ?
3.5.1.2 L-gamma-glutamyl-anilide + H2O
-
 Pseudomonas aeruginosa L-Glu + aniline
-
 ?
3.5.1.2 L-gamma-glutamyl-anilide + hydroxylamine
-
 Pseudomonas aeruginosa L-gamma-glutamyl hydroxamate + aniline
-
 ?
3.5.1.2 L-gamma-glutamyl-ethylester + H2O
-
 Pseudomonas aeruginosa L-Glu + ethanol
-
 ?
3.5.1.2 L-gamma-glutamyl-hydrazide + H2O
-
 Pseudomonas aeruginosa L-Glu + hydroxyhydrazine
-
 ?
3.5.1.2 L-gamma-glutamyl-hydrazide + hydroxylamine
-
 Pseudomonas aeruginosa L-gamma-glutamyl hydroxamate + hydroxyhydrazine
-
 ?
3.5.1.2 L-gamma-glutamyl-L-Ala + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.5.1.2 L-gamma-glutamyl-L-Ala + hydroxylamine
-
 Pseudomonas aeruginosa gamma-glutamyl hydroxamate + Ala
-
 ?
3.5.1.2 L-gamma-glutamyl-L-epsilon-Lys + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.5.1.2 L-gamma-glutamyl-L-epsilon-Lys + hydroxylamine
-
 Pseudomonas aeruginosa ?
-
 ?
3.5.1.2 L-gamma-glutamyl-L-Glu + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.5.1.2 L-gamma-glutamyl-L-Glu + hydroxylamine
-
 Pseudomonas aeruginosa ?
-
 ?
3.5.1.2 L-gamma-glutamyl-methylester + H2O
-
 Pseudomonas aeruginosa L-Glu + methanol
-
 ?
3.5.1.2 L-gamma-glutamyl-methylester + hydroxylamine
-
 Pseudomonas aeruginosa L-gamma-glutamyl hydroxamate + methanol
-
 ?
3.5.1.2 L-Gln + H2O glutaminase A and B Pseudomonas aeruginosa L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + hydroxylamine glutaminase A and B Pseudomonas aeruginosa L-gamma-glutamyl hydroxamate + NH3
-
 ?
3.5.1.2 L-theanine + H2O
-
 Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 tetramer 4 * 67000, glutaminase B, SDS-PAGE Pseudomonas aeruginosa
3.5.1.2 tetramer 4 * 35000, glutaminase A, SDS-PAGE Pseudomonas aeruginosa