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Literature summary extracted from

  • Holcenberg, J.S.
    Glutaminase from Acinetobacter glutaminsificans (1985), Methods Enzymol., 113, 257-263.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.2
-
 Acinetobacter glutaminasificans

General Stability

EC Number General Stability Organism
3.5.1.2 freezing and thawing produces a substantial loss of activity Acinetobacter glutaminasificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 6-diazo-5-oxo-L-norleucine
-
 Acinetobacter glutaminasificans
3.5.1.2 glutamate no inhibition Acinetobacter glutaminasificans
3.5.1.2 methylene blue 0.005%, irreversible inactivation Acinetobacter glutaminasificans
3.5.1.2 Rose bengal 0.01%, irreversible inactivation Acinetobacter glutaminasificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 4.8
-
 L-Asn
-
 Acinetobacter glutaminasificans
3.5.1.2 5.8
-
 L-Gln
-
 Acinetobacter glutaminasificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 33000
-
 4 * 33000 Acinetobacter glutaminasificans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Acinetobacter glutaminasificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2
-
 Acinetobacter glutaminasificans

Storage Stability

EC Number Storage Stability Organism
3.5.1.2 5°C, enzyme preparation as a lyophilized powder, indefinitely stable Acinetobacter glutaminasificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 beta-cyanoalanine + H2O 0.2% of the activity with L-Gln Acinetobacter glutaminasificans ?
-
 ?
3.5.1.2 D-Asn + H2O 26% of the activity with L-Gln Acinetobacter glutaminasificans D-Asp + NH3
-
 ?
3.5.1.2 D-Gln + H2O 35% of the activity with L-Gln Acinetobacter glutaminasificans D-Glu + NH3
-
 ?
3.5.1.2 Gln + H2O
-
 Acinetobacter glutaminasificans Glu + NH3
-
 ?
3.5.1.2 L-Asn + H2O 77% of the activity with L-Gln Acinetobacter glutaminasificans L-Asp + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 tetramer 4 * 33000 Acinetobacter glutaminasificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 7
-
 hydrolysis of L-Asn and L-Gln Acinetobacter glutaminasificans