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Literature summary extracted from

  • Hoagland, R.E.; Graf, G.
    The purification and properties of an amidohydrolase from soybean (1974), Can. J. Biochem., 52, 903-910.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.13 Ag+ 0.5 mM AgCl, complete inhibition Glycine max
3.5.1.13 catechol 0.5 mM, 8.5% inhibition Glycine max
3.5.1.13 Cu2+ 0.5 mM CuSO4, 65.5% loss of activity Glycine max
3.5.1.13 HgCl2
-
 Glycine max
3.5.1.13 NEM
-
 Glycine max
3.5.1.13 Ni2+ 0.2 mM NiCl2, 50.2% inhibition Glycine max
3.5.1.13 p-benzoquinone
-
 Glycine max
3.5.1.13 PCMB
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.13 0.0578
-
 Nalpha-benzoyl-DL-Arg 2-naphthylamide
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.13 63000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.13 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.13
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.13 root roots of seedlings Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.13 0.81
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
3.5.1.13 -15°C, stable for several months Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.13 Gly-2-naphthylamide + H2O
-
 Glycine max Gly 2-naphthylamine
-
 ?
3.5.1.13 Gly-p-nitroanilide + H2O
-
 Glycine max Gly + p-nitroaniline
-
 ?
3.5.1.13 L-Ala beta-naphthylamide + H2O
-
 Glycine max Ala + beta-naphthylamine
-
 ?
3.5.1.13 L-Arg beta-naphthylamide + H2O
-
 Glycine max Arg + beta-naphthylamine
-
 ?
3.5.1.13 L-Leu beta-naphthylamide + H2O
-
 Glycine max Leu + beta-naphthylamine
-
 ?
3.5.1.13 L-Leu p-nitroanilide + H2O
-
 Glycine max Leu + p-nitroaniline
-
 ?
3.5.1.13 L-Leu-L-Ala + H2O
-
 Glycine max Leu + Ala
-
 ?
3.5.1.13 L-Leu-L-Phe + H2O
-
 Glycine max Leu + Phe
-
 ?
3.5.1.13 L-Leu-L-Tyr + H2O
-
 Glycine max Leu + Tyr
-
 ?
3.5.1.13 L-Leu-L-Val + H2O
-
 Glycine max Leu + Val
-
 ?
3.5.1.13 L-Lys beta-naphthylamide + H2O
-
 Glycine max Lys + beta-naphthylamine
-
 ?
3.5.1.13 L-Met beta-naphthylamide + H2O
-
 Glycine max Met + beta-naphthylamine
-
 ?
3.5.1.13 Nalpha-benzoyl-DL-Arg beta-naphthylamide + H2O
-
 Glycine max Nalpha-benzoyl-DL-Arg + beta-naphthylamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.13 50
-
 hydrolysis of Nalpha-benzoyl-DL-Arg p-nitroanilide Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.13 45
-
 5 min, stable up to Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.13 8
-
-
 Glycine max