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Literature summary extracted from

  • Tsavdaridis, I.K.; Triantafillidou, D.C.; Kyriakidis, D.A.
    Two forms of L-asparaginase in Tetrahymena thermophila (1994), Biochem. Mol. Biol. Int., 32, 67-77.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 albumin 0.1 mg/ml, 3fold activation of L-asparaginase II Tetrahymena thermophila
3.5.1.1 sorbitol 30% w/v, activates more than 10fold Tetrahymena thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Phospholipase C
-
 Tetrahymena thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 4.1
-
 L-Asn asparaginase I and II Tetrahymena thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 rough endoplasmic reticulum
-
 Tetrahymena thermophila 5791
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Ca2+ 5 mM, 40-45% activation of L-asparaginase I and II Tetrahymena thermophila
3.5.1.1 Mg2+ 5-10% activation Tetrahymena thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 50000
-
 x * 50000, L-asparaginase I, SDS-PAGE Tetrahymena thermophila
3.5.1.1 93000
-
 x * 93000, L-asparaginase II, SDS-PAGE Tetrahymena thermophila
3.5.1.1 200000
-
 L-asparaginase II, gel filtration Tetrahymena thermophila
3.5.1.1 1000000
-
 L-asparaginase I, gel filtration Tetrahymena thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Tetrahymena thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 L-asparaginase I and II Tetrahymena thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 36.1
-
-
 Tetrahymena thermophila
3.5.1.1 59.6
-
-
 Tetrahymena thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-Asn + H2O
-
 Tetrahymena thermophila L-Asp + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 93000, L-asparaginase II, SDS-PAGE Tetrahymena thermophila
3.5.1.1 ? x * 50000, L-asparaginase I, SDS-PAGE Tetrahymena thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8.6
-
 L-asparaginase I and II Tetrahymena thermophila