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Literature summary extracted from

  • Joner, P.E.; Kristiansen, T.; Einasson, M.
    Purification and properties of L-asparaginase A from Acinetobacter calcoaceticus (1973), Biochim. Biophys. Acta, 327, 146-156.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Co2+
-
 Acinetobacter calcoaceticus
3.5.1.1 Cu2+
-
 Acinetobacter calcoaceticus
3.5.1.1 Hg2+
-
 Acinetobacter calcoaceticus
3.5.1.1 NH4+
-
 Acinetobacter calcoaceticus
3.5.1.1 Ni2+
-
 Acinetobacter calcoaceticus
3.5.1.1 PCMB reversed by mercaptoethanol Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 2
-
 L-asparagine
-
 Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ stimulates Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 25000
-
 4 * 25000, SDS-PAGE Acinetobacter calcoaceticus
3.5.1.1 105000
-
 gel filtration Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 32
-
-
 Acinetobacter calcoaceticus

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -20°C, little loss of activity after prolonged storage Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-Asn + H2O at 5% of the activity with L-Asn Acinetobacter calcoaceticus D-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Acinetobacter calcoaceticus L-Asp + NH3
-
 ?
3.5.1.1 L-Gln + H2O at 10% of the activity with L-Asn Acinetobacter calcoaceticus L-Glu + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 tetramer 4 * 25000, SDS-PAGE Acinetobacter calcoaceticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 30
-
 3 h, stable Acinetobacter calcoaceticus
3.5.1.1 60
-
 5 min, complete inactivation Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8.4 8.8
-
 Acinetobacter calcoaceticus