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Literature summary extracted from
- Purification and characterization of L-asparaginase with anti-lymphoma activity from Vibrio succinogenes (1976), J. Biol. Chem., 251, 6929-6933.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 0.0478 | - |
L-asparagine | - |
Wolinella succinogenes |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 37000 | - |
x * 37000, SDS-PAGE | Wolinella succinogenes |
| 3.5.1.1 | 146000 | - |
- |
Wolinella succinogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Wolinella succinogenes | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | no modification | no carbohydrate and phosphorus detected | Wolinella succinogenes |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | - |
Wolinella succinogenes |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 202 | - |
- |
Wolinella succinogenes |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.1 | -20°C, stable for at least 3 months | Wolinella succinogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | D-Asn + H2O | 6.5% of the activity with L-Asn | Wolinella succinogenes | D-Asp + NH3 | - |
? | |
| 3.5.1.1 | L-Asn + H2O | - |
Wolinella succinogenes | L-Asp + NH3 | - |
? | |
| 3.5.1.1 | L-aspartic acid beta-hydroxamate + H2O | - |
Wolinella succinogenes | L-Asp + hydroxylamine | - |
? | |
| 3.5.1.1 | L-Gln + H2O | not hydrolyzed at significant rate | Wolinella succinogenes | L-Glu + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | ? | x * 37000, SDS-PAGE | Wolinella succinogenes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 7.3 | - |
- |
Wolinella succinogenes |
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