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Literature summary extracted from
- Purification and properties of L-asparaginase B from Acinetobacter calcoaceticus (1976), Biochim. Biophys. Acta, 438, 287-295.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | citrate | stimulates | Acinetobacter calcoaceticus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.1.1 | freezing at -20°C, complete and irreversible loss of activity | Acinetobacter calcoaceticus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | KCl | - |
Acinetobacter calcoaceticus | |
| 3.5.1.1 | Na2SO4 | - |
Acinetobacter calcoaceticus | |
| 3.5.1.1 | NaCl | - |
Acinetobacter calcoaceticus | |
| 3.5.1.1 | NaN3 | - |
Acinetobacter calcoaceticus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 3 | - |
L-Asn | - |
Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 130000 | - |
gel filtration | Acinetobacter calcoaceticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Acinetobacter calcoaceticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | - |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 70 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.1 | 4°C, pH 6.0, 0.1 M citrate buffer, stable for several weeks | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | D-Asn + H2O | 30% of the activity with L-Asn | Acinetobacter calcoaceticus | D-Asp + NH3 | - |
? | |
| 3.5.1.1 | L-Asn + H2O | - |
Acinetobacter calcoaceticus | L-Asp + NH3 | - |
? | |
| 3.5.1.1 | L-Gln + H2O | to about the same extent as L-Asn | Acinetobacter calcoaceticus | L-Glu + NH3 | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 50 | - |
3 h, little loss of activity | Acinetobacter calcoaceticus |
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