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Literature summary extracted from

  • Chang, C.; Gill, S.S.
    Purification and characterization of an epoxide hydrolase from the peroxisomal fraction of mouse liver (1991), Arch. Biochem. Biophys., 285, 276-284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 4'-fluorochalcone oxide
-
 Mus musculus
3.3.2.10 4'-methylchalcone oxide
-
 Mus musculus
3.3.2.10 4-benzyloxychalcone oxide
-
 Mus musculus
3.3.2.10 4-fluorochalcone oxide
-
 Mus musculus
3.3.2.10 4-methylchalcone oxide
-
 Mus musculus
3.3.2.10 acetonitrile 89% loss of activity at 5% acetonitrile Mus musculus
3.3.2.10 beta-naphthoflavone
-
 Mus musculus
3.3.2.10 chalcone
-
 Mus musculus
3.3.2.10 Chalcone oxide
-
 Mus musculus
3.3.2.10 Clofibrate 2 mM, 70-100% inhibition Mus musculus
3.3.2.10 ethanol 3% inhibition at 3% ethanol Mus musculus
3.3.2.10 styrene oxide
-
 Mus musculus
3.3.2.10 tetrahydrofuran 73% loss of activity with 1% tetrahydrofuran, 98% loss of activity with 5% tetrahydrofuran Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 0.0052
-
 trans-stilbene oxide cytosolic enzyme Mus musculus
3.3.2.10 0.006
-
 trans-stilbene oxide peroxisomal enzyme Mus musculus
3.3.2.10 0.027
-
 cis-stilbene oxide peroxisomal enzyme Mus musculus
3.3.2.10 0.076
-
 cis-stilbene oxide cytosolic enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 peroxisome
-
 Mus musculus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 57000
-
 gel filtration Mus musculus
3.3.2.10 59000
-
 1 * 59000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.10 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.10 0.167
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 cis-stilbene oxide + H2O
-
 Mus musculus ?
-
 ?
3.3.2.10 trans-stilbene oxide + H2O
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.10 monomer 1 * 59000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.3.2.10 TSO hydrolase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 51
-
-
 Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.3.2.10 37 51 37°C: about 60% of maximal activity, 51°C: optimum Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 6.8
-
-
 Mus musculus