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Literature summary extracted from

  • De Roover, J.; Van Laere, A.; De Winter, M.; Timmermanns, J.W.; Van den Ende, W.
    Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybus (1999), Physiol. Plant., 106, 28-34.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 CaCl2 partially Cichorium intybus
3.2.1.80 EDTA partially Cichorium intybus
3.2.1.80 sucrose
-
 Cichorium intybus
3.2.1.153 CaCl2 1 mM, partial Cichorium intybus
3.2.1.153 Na-EDTA 1 mM, partial Cichorium intybus
3.2.1.153 sucrose strong Cichorium intybus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.153 58
-
 1-kestose
-
 Cichorium intybus
3.2.1.153 64
-
 1,1-nystose
-
 Cichorium intybus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 60000
-
 gel-filtration Cichorium intybus
3.2.1.80 64000
-
 SDS-PAGE Cichorium intybus
3.2.1.153 60000
-
 gel filtration Cichorium intybus
3.2.1.153 64000
-
 1 * 64000, SDS-PAGE Cichorium intybus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Cichorium intybus
-
-
-
3.2.1.153 Cichorium intybus
-
 L. var. foliosum cv. Flash
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Cichorium intybus
3.2.1.153
-
 Cichorium intybus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.153 root
-
 Cichorium intybus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Cichorium intybus D-fructose + ?
-
 ?
3.2.1.153 1,1-nystose + H2O
-
 Cichorium intybus ?
-
 ?
3.2.1.153 1-kestose + H2O
-
 Cichorium intybus ?
-
 ?
3.2.1.153 inulin + H2O
-
 Cichorium intybus fructose + ?
-
 ?
3.2.1.153 additional information the enzyme hydrolyzes the beta(2-1) linkage in inulin, 1-kestose and 1,1-nystose at rates at least 5fold faster than the beta(2-6) linkages in levan oligosaccharides and levanbiose Cichorium intybus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.153 monomer 1 * 64000, SDS-PAGE Cichorium intybus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.153 1-fructan exohydrolase
-
 Cichorium intybus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 35
-
 inulin Cichorium intybus
3.2.1.153 35
-
-
 Cichorium intybus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5 5.5 inulin Cichorium intybus
3.2.1.153 5 5.5
-
 Cichorium intybus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.153 5.9
-
 sucrose
-
 Cichorium intybus