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Literature summary extracted from

  • Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.
    Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans (1987), J. Bacteriol., 169, 4507-4517.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expression in Escherichia coli Streptococcus mutans
3.2.1.154 fruA is expressed under its own control in Escherichia coli JM83(pFRU1) Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Ag+
-
 Streptococcus mutans
3.2.1.80 Cu2+ partially Streptococcus mutans
3.2.1.80 Hg2+
-
 Streptococcus mutans
3.2.1.154 Ag2+ 1 mM, almost complete inhibition Streptococcus mutans
3.2.1.154 Cu2+ 1 mM, about 20% inhibition Streptococcus mutans
3.2.1.154 Hg2+ 0.01 mM, almost complete inhibition Streptococcus mutans
3.2.1.154 Mg2+ 1 mM, about 20% inhibition Streptococcus mutans
3.2.1.154 additional information no inhibition by F- or Tris. No evidence of product inhibition by fructose Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.154 cytoplasm
-
 Streptococcus mutans 5737
-
3.2.1.154 cytoplasmic membrane
-
 Streptococcus mutans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.80 Co2+ slight stimulation Streptococcus mutans
3.2.1.80 Mn2+ slight stimulation Streptococcus mutans
3.2.1.154 Co2+ slight stimulation Streptococcus mutans
3.2.1.154 Mn2+ slight stimulation Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 140000
-
 x * 140000, SDS-PAGE Streptococcus mutans
3.2.1.154 140000
-
 x * 140000, SDS-PAGE Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.80 fructans + H2O Streptococcus mutans degradation D-fructose
-
 ?
3.2.1.80 fructans + H2O Streptococcus mutans GS-5 degradation D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Streptococcus mutans
-
 GS-5
-
3.2.1.80 Streptococcus mutans GS-5
-
 GS-5
-
3.2.1.154 Streptococcus mutans
-
 GS-5
-
3.2.1.154 Streptococcus mutans GS-5
-
 GS-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Streptococcus mutans
3.2.1.154
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.154 66.4
-
 enzyme from Streptococcus mutans Streptococcus mutans
3.2.1.154 204.5
-
 recombinant enzyme expressed in Escherichia coli Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 fructans + H2O degradation Streptococcus mutans D-fructose
-
 ?
3.2.1.80 fructans + H2O degradation Streptococcus mutans GS-5 D-fructose
-
 ?
3.2.1.80 inulin + H2O
-
 Streptococcus mutans D-fructose + ?
-
 ?
3.2.1.80 inulin + H2O
-
 Streptococcus mutans GS-5 D-fructose + ?
-
 ?
3.2.1.80 levan + H2O
-
 Streptococcus mutans D-fructose + ?
-
 ?
3.2.1.80 levan + H2O
-
 Streptococcus mutans GS-5 D-fructose + ?
-
 ?
3.2.1.80 raffinose + H2O
-
 Streptococcus mutans D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
 ?
3.2.1.80 raffinose + H2O
-
 Streptococcus mutans GS-5 D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
 ?
3.2.1.80 sucrose + H2O
-
 Streptococcus mutans D-fructose + D-glucose
-
 ?
3.2.1.80 sucrose + H2O
-
 Streptococcus mutans GS-5 D-fructose + D-glucose
-
 ?
3.2.1.154 inulin + H2O 34% of the activity with levan Streptococcus mutans fructose + ?
-
 ?
3.2.1.154 levan + H2O
-
 Streptococcus mutans fructose + ?
-
 ?
3.2.1.154 raffinose + H2O 12% of the activity with levan Streptococcus mutans ?
-
 ?
3.2.1.154 sucrose + H2O 21% of the activity with levan Streptococcus mutans D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 ? x * 140000, SDS-PAGE Streptococcus mutans
3.2.1.154 ? x * 140000, SDS-PAGE Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 4.5
-
 sucrose Streptococcus mutans
3.2.1.80 5.5
-
 fructan polymers Streptococcus mutans
3.2.1.154 4.5
-
 sucrose degradation Streptococcus mutans
3.2.1.154 5.5
-
 reaction with levan or inulin Streptococcus mutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.80 3.5 7 little fructanase activity at or above pH 7.0 Streptococcus mutans
3.2.1.154 4 6 pH 4.0: about 85% of maximal activity, pH 6.0: about 70% of maximal activity, reaction with sucrose Streptococcus mutans
3.2.1.154 4 7 pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity, reaction with levan or inulin Streptococcus mutans