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Literature summary extracted from

  • Akino, T.; Nakamura, N.; Horikoshi, K.
    Characterization of three beta-mannanases of an alkalophilic Bacillus sp. (1988), Agric. Biol. Chem., 52, 773-779.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 cysteine slight activation Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information copra beta-mannan: 2.0 mg/ml, locust bean beta-mannan: 3.8 mg/ml, konjak beta-mannan: 7.7 mg/ml, carob galactomannan: 0.9 mg/ml Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 42000
-
 M-III, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.78 58000
-
 M-I, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.78 59000
-
 M-II, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus sp. (in: Bacteria)
-
 M-I, M-II, M-III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 M-I, M-II, M-III Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 endosperm
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 470
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 galactoglucomannan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Bacillus sp. (in: Bacteria) oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Bacillus sp. (in: Bacteria) oligosaccharides
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Bacillus sp. (in: Bacteria) mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannotetraose + H2O
-
 Bacillus sp. (in: Bacteria) mannobiose + mannose + mannotriose small amounts of mannose and mannotriose ?
3.2.1.78 mannotriose + H2O
-
 Bacillus sp. (in: Bacteria) mannobiose + mannose
-
 ?
3.2.1.78 additional information not: mannobiose Bacillus sp. (in: Bacteria) ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 60
-
 M-I, M-II Bacillus sp. (in: Bacteria)
3.2.1.78 65
-
 M-III Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 50 70
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 8.5
-
 M-III, at 65°C Bacillus sp. (in: Bacteria)
3.2.1.78 9
-
 M-I, M-II, at 60°C Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.78 6 10
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus sp. (in: Bacteria)