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Literature summary extracted from

  • Sugiura, M.; Ito, A.; Yamaguchi, T.
    Studies on dextranase. II. New exo-dextranase from Brevibacterium fuscum var. Dextranlyticum (1974), Biochim. Biophys. Acta, 350, 61-70.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.70 Cu2+
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.70 Hg2+
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.70 Iodine
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.70 N-bromosuccinimide
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.70 p-chloromercuribenzoate
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.95 AgNO3 2 mM, 97% inhibition Brevibacterium fuscum
3.2.1.95 CuSO4 2 mM, 76% inhibition Brevibacterium fuscum
3.2.1.95 HgCl2 2 mM, complete inhibition Brevibacterium fuscum
3.2.1.95 Iodine 2 mM, complete inhibition Brevibacterium fuscum
3.2.1.95 N-bromosuccinimide 2 mM, complete inhibition Brevibacterium fuscum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.70 dextrans + H2O Brevibacterium fuscum var. dextranlyticum
-
 beta-D-glucose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.70 Brevibacterium fuscum var. dextranlyticum
-
-
-
3.2.1.95 Brevibacterium fuscum
-
 var. dextranlyticum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.70
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.95
-
 Brevibacterium fuscum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.70 34.3 38.4
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.95 additional information
-
-
 Brevibacterium fuscum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.70 dextrans + H2O
-
 Brevibacterium fuscum var. dextranlyticum beta-D-glucose
-
 ir
3.2.1.70 isomaltodextrins + H2O
-
 Brevibacterium fuscum var. dextranlyticum beta-D-glucose
-
 ir
3.2.1.95 dextran + H2O
-
 Brevibacterium fuscum isomaltotriose
-
 ?
3.2.1.95 isomaltodextrin + H2O reduced Brevibacterium fuscum isomaltotriose
-
 ?
3.2.1.95 Sephadex G-100 + H2O
-
 Brevibacterium fuscum ?
-
 ?
3.2.1.95 Sephadex G-200 + H2O
-
 Brevibacterium fuscum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.70 55
-
 not stable above Brevibacterium fuscum var. dextranlyticum
3.2.1.95 50
-
 pH 7.5, 30 min, stable up to Brevibacterium fuscum
3.2.1.95 60
-
 pH 7.5, 30 min, 30% loss of activity Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.95 7 7.5
-
 Brevibacterium fuscum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.95 6.5 8 85% of maximum activity at pH 6.5 and pH 8.0 Brevibacterium fuscum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.70 5 11
-
 Brevibacterium fuscum var. dextranlyticum
3.2.1.95 5.6 11 37°C, 12 h, stable Brevibacterium fuscum