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Literature summary extracted from

  • Kraml, J.; Kolinska, J.; Ellederova, D.; Hirsova, D.
    beta-Glucosidase (phlorizin hydrolase) activity of the lactase fraction isolated from the small intestinal mucosa of infant rats, and the relationship between beta-glucosidases and beta-galactosidases (1972), Biochim. Biophys. Acta, 258, 520-530.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 D-galactono-1,4-lactone competitive inhibition of lactase activity, Ki: 0.35 mM Rattus sp.
3.2.1.62 D-glucono-1,5-lactone competitive inhibition of lactase activity, Ki: 0.027 mM Rattus sp.
3.2.1.62 phlorizin competitive inhibition of lactase activity, Ki: 0.047 mM Rattus sp.
3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 4.5 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.4
-
 phlorizin
-
 Rattus sp.
3.2.1.62 0.4
-
 phloretin-beta-D-galactoside
-
 Rattus sp.
3.2.1.62 1.1
-
 4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
 Rattus sp.
3.2.1.62 4.1
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rattus sp.
3.2.1.62 13.1
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Rattus sp.
3.2.1.62 22.3
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Rattus sp.
3.2.1.62 40
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 brush border
-
 Rattus sp. 5903
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp. best substrate D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O Rattus sp. cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Rattus sp.
-
 phloretin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 partial Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine
-
 Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Rattus sp. 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Rattus sp. D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rattus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.62 phloretin-beta-D-galactoside + H2O very low activity Rattus sp. phloretin + beta-D-galactose
-
 ?
3.2.1.62 phlorizin + H2O
-
 Rattus sp. phloretin + beta-D-glucose
-
 ?