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Literature summary extracted from

  • Skovbjerg, H.; Noren, O.; Sjöström, H.; Danielsen, E.M.; Enevoldsen, B.S.
    Further characterization of intestinal lactase/phlorizin hydrolase (1982), Biochim. Biophys. Acta, 707, 89-97.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 phlorizin competitive inhibition of lactase activity Homo sapiens
3.2.1.62 phlorizin competitive inhibition of lactase activity, Ki: 0.37 mM Sus scrofa
3.2.1.62 Tris(hydroxymethyl)aminomethane
-
 Homo sapiens
3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 5.1 mM Sus scrofa
3.2.1.108 phlorizin
-
 Homo sapiens
3.2.1.108 phlorizin competitive inhibitor of lactose hydrolysis Sus scrofa
3.2.1.108 Tris competitive Homo sapiens
3.2.1.108 Tris competitive Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 5.1
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside slightly higher catalytic efficiency than for the human enzyme Sus scrofa
3.2.1.108 5.1
-
 lactose
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 microvillus
-
 Homo sapiens 5902
-
3.2.1.62 microvillus
-
 Sus scrofa 5902
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.62 160000
-
 2 * 160000, SDS-PAGE Sus scrofa
3.2.1.62 320000
-
 gel filtration, amphiphilic form Homo sapiens
3.2.1.62 320000
-
 gel filtration, amphiphilic form Sus scrofa
3.2.1.108 160000
-
 2 * 160000, SDS-PAGE Homo sapiens
3.2.1.108 160000
-
 2 * 160000, SDS-PAGE Sus scrofa
3.2.1.108 320000
-
-
 Homo sapiens
3.2.1.108 320000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Homo sapiens
-
 D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Sus scrofa two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 cellotetraose + H2O Homo sapiens considerable activity ? + beta-D-glucose
-
 ?
3.2.1.62 cellotetraose + H2O Sus scrofa considerable activity ? + beta-D-glucose
-
 ?
3.2.1.62 cellotriose + H2O Homo sapiens considerable activity ? + beta-D-glucose
-
 ?
3.2.1.62 cellotriose + H2O Sus scrofa considerable activity ? + beta-D-glucose
-
 ?
3.2.1.62 cellulose + H2O Homo sapiens very low activity ? + beta-D-glucose
-
 ?
3.2.1.62 cellulose + H2O Sus scrofa very low activity ? + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Homo sapiens
-
 phloretin + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Sus scrofa 5% of the activity of lactose hydrolysis phloretin + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Sus scrofa hydrolysis located on a specific active site for phlorizin hydrolysis phloretin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Homo sapiens
-
-
-
3.2.1.62 Sus scrofa
-
-
-
3.2.1.108 Homo sapiens
-
-
-
3.2.1.108 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 to homogeneity, immunoadsorbent chromatography, amphiphilic form Sus scrofa
3.2.1.108
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine small intestine Homo sapiens
-
3.2.1.62 intestine small intestine Sus scrofa
-
3.2.1.62 intestine proximal jejenum Sus scrofa
-
3.2.1.108 small intestine
-
 Homo sapiens
-
3.2.1.108 small intestine
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.108 18
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 2-naphthyl-beta-D-galactopyranoside + H2O very low activity Sus scrofa 2-naphthol + beta-D-galactopyranose
-
 ?
3.2.1.62 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Homo sapiens 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 2-nitrophenyl beta-D-galactopyranoside + H2O lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Sus scrofa 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Sus scrofa 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Sus scrofa 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
 Homo sapiens D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis Sus scrofa D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis Sus scrofa D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Sus scrofa beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-bromonaphthyl-beta-D-galactopyranoside very low activity Sus scrofa 6-bromonaphthol + beta-D-galactopyranose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Sus scrofa beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Sus scrofa beta-D-glucose + D-glucose
-
 ?
3.2.1.62 cellotetraose + H2O considerable activity Homo sapiens ? + beta-D-glucose
-
 ?
3.2.1.62 cellotetraose + H2O considerable activity Sus scrofa ? + beta-D-glucose
-
 ?
3.2.1.62 cellotriose + H2O considerable activity Homo sapiens ? + beta-D-glucose
-
 ?
3.2.1.62 cellotriose + H2O considerable activity Sus scrofa ? + beta-D-glucose
-
 ?
3.2.1.62 cellulose + H2O very low activity Homo sapiens ? + beta-D-glucose
-
 ?
3.2.1.62 cellulose + H2O very low activity Sus scrofa ? + beta-D-glucose
-
 ?
3.2.1.62 phenyl-beta-D-galactopyranoside + H2O low activity Sus scrofa phenol + beta-D-galactopyranose
-
 ?
3.2.1.62 phlorizin + H2O
-
 Homo sapiens phloretin + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O 5% of the activity of lactose hydrolysis Sus scrofa phloretin + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O hydrolysis located on a specific active site for phlorizin hydrolysis Sus scrofa phloretin + beta-D-glucose
-
 ?
3.2.1.108 cellobiose + H2O 25% of the activity with lactose Sus scrofa 2 beta-D-glucose
-
 ?
3.2.1.108 cellobiose + H2O 14% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
 ?
3.2.1.108 cellotetraose + 3 H2O 14% of the activity with lactose Sus scrofa 4 beta-D-glucose
-
 ?
3.2.1.108 cellotetraose + 3 H2O 9% of the activity with lactose Homo sapiens 4 beta-D-glucose
-
 ?
3.2.1.108 cellotriose + H2O 14% of the activity with lactose Homo sapiens 3 D-glucose
-
 ?
3.2.1.108 cellotriose + H2O 9% of the activity with lactose Sus scrofa 3 D-glucose
-
 ?
3.2.1.108 cellulose + H2O 0.1% of the activity with lactose Homo sapiens beta-D-glucose
-
 ?
3.2.1.108 cellulose + H2O 0.1% of the activity with lactose Sus scrofa beta-D-glucose
-
 ?
3.2.1.108 lactose + H2O
-
 Homo sapiens D-glucose + D-galactose
-
 ?
3.2.1.108 lactose + H2O
-
 Sus scrofa D-glucose + D-galactose
-
 ?
3.2.1.108 phlorizin + H2O
-
 Homo sapiens phloretin + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.62 dimer
-
 Homo sapiens
3.2.1.62 dimer 2 * 160000, SDS-PAGE Sus scrofa
3.2.1.108 dimer 2 * 160000, SDS-PAGE Homo sapiens
3.2.1.108 dimer 2 * 160000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.62 49
-
 lactase activity faster destroyed than phlorizin activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.62 5.6 6.2
-
 Sus scrofa
3.2.1.108 5.6 6.2
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.108 4 10 pH 4.0: about 60% of maximal activity, pH 10.0: about 50% of maximal activity Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.62 3.2
-
 little activity below Sus scrofa