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Literature summary extracted from

  • Reglero, A.; Cabezas, J.A.
    Glycosidases of molluscs. Purification and properties of alpha-L-fucosidase from Chamelea gallina L. (1976), Eur. J. Biochem., 66, 379-387.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 CN- 30% activation at 10 mM Chamelea gallina
3.2.1.51 ethylenediaminetetraacetic acid 5% activation at 10 mM Chamelea gallina

General Stability

EC Number General Stability Organism
3.2.1.51 sensitive to repeated freezing and thawing Chamelea gallina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose competitive inhibition at 50 mM Chamelea gallina
3.2.1.51 D-fucose 15% inhibition at 50 mM Chamelea gallina
3.2.1.51 D-galactono-1,4-lactone 15% inhibition at 50 mM Chamelea gallina
3.2.1.51 D-galactose 15% inhibition at 50 mM Chamelea gallina
3.2.1.51 D-glucono-1,5-lactone 30% inhibition at 50 mM Chamelea gallina
3.2.1.51 D-glucose 15% inhibition at 50 mM Chamelea gallina
3.2.1.51 Hg2+ effective non-competitive inhibition at 2 mM Chamelea gallina
3.2.1.51 L-galactono-1,4-lactone 30% inhibition at 50 mM Chamelea gallina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.07
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Chamelea gallina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Ca2+ 10% activation at 10 mM Chamelea gallina
3.2.1.51 Zn2+ 10% activation at 10 mM Chamelea gallina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 200000
-
 gel filtration Chamelea gallina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 Fuc-alpha-(1-2)-Gal + H2O Chamelea gallina
-
 alpha-L-fucose + D-galactose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Chamelea gallina 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Chamelea gallina 3-fucosyllactose alpha-D-fucose + lactose
-
 ?
3.2.1.51 Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Chamelea gallina lacto-N-fucopentaose III alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O Chamelea gallina
-
 alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O Chamelea gallina
-
 alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 oligosaccharide + H2O Chamelea gallina di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O Chamelea gallina
-
 alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O Chamelea gallina
-
 alpha-L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Chamelea gallina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, isoelectric focusing Chamelea gallina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 hepatopancreas
-
 Chamelea gallina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 1620
-
-
 Chamelea gallina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages Chamelea gallina 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal + H2O
-
 Chamelea gallina alpha-L-fucose + D-galactose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Chamelea gallina alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Chamelea gallina alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Chamelea gallina alpha-D-fucose + lactose
-
 ?
3.2.1.51 Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose III Chamelea gallina alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
-
 Chamelea gallina alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O
-
 Chamelea gallina alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 oligosaccharide + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 oligosaccharides + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O
-
 Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O
-
 Chamelea gallina alpha-L-fucose + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 65
-
 fully active after 5 min Chamelea gallina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.2
-
-
 Chamelea gallina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 4 6 stable between Chamelea gallina