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Literature summary extracted from

  • Grove, D.S.; Serif, G.S.
    Porcine thyroid fucosidase (1981), Biochim. Biophys. Acta, 662, 246-255.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate complete inactivation at 22 mM Sus scrofa
3.2.1.51 4-mercuribenzoate complete inhibition at 0.25 mM Sus scrofa
3.2.1.51 alpha-L-fucose competitive inhibition, Ki: 0.13 mM Sus scrofa
3.2.1.51 D-arabinose competitive inhibition, Ki: 50 mM Sus scrofa
3.2.1.51 D-galactose competitive inhibition, Ki: 1.7 mM Sus scrofa
3.2.1.51 L-rhamnose competitive inhibition, Ki: 140 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.3
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 55000
-
 4 * 55000, SDS-PAGE Sus scrofa
3.2.1.51 192000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O Sus scrofa
-
 alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Sus scrofa lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O Sus scrofa
-
 alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 thyroglobulin + H2O Sus scrofa hydrolysis only after pronase or neuraminidase treatment alpha-L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein
-
 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 7.8
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
 Sus scrofa 4-methylumbelliferone + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Sus scrofa 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
-
 Sus scrofa alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Sus scrofa alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O
-
 Sus scrofa alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 submaxilary mucin trisaccharide + H2O
-
 Sus scrofa alpha-L-fucose + disaccharide
-
 ?
3.2.1.51 thyroglobulin + H2O hydrolysis only after pronase or neuraminidase treatment Sus scrofa alpha-L-fucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 55000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.1
-
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 140
-
 L-rhamnose competitive inhibition Sus scrofa