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Literature summary extracted from

  • Zhao, K.W.; Neufeld, E.F.
    Purification and characterization of recombinant human alpha-N-acetylglucosaminidase secreted by chinese hamster ovary cells (2000), Protein Expr. Purif., 19, 202-211.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.50 expressed in Chinese hamster ovary cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.50 2-acetamido-1,2-dideoxynojirimycin competitive inhibition Homo sapiens
3.2.1.50 6-acetamido-6-deoxycastanospermine competitive inhibition Homo sapiens
3.2.1.50 iodoacetate complete inhibition at 2.5 mM Homo sapiens
3.2.1.50 p-chloromercuribenzoate complete inhibition at 0.005 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.22
-
methylumbelliferyl-N-acetyl-alpha-D-glucosaminide purified enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.50 Hg2+ complete inhibition at 0.0015 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 83000
-
SDS-PAGE Homo sapiens
3.2.1.50 90000
-
2 * 90000, monomer of 90000 and dimer in a ratio 1:2, gel filtration Homo sapiens
3.2.1.50 90000 180000 monomer of 90000 and dimer of 180000 in the ratio 1:2, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Homo sapiens involved in the degradation of heparin and heparan sulfate (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.50 glycoprotein naturally occuring enzyme contains mannose-6-phosphate Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 ovary
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 0.58
-
purified enzyme, methylumbelliferyl-N-acetyl-alpha-D-glucosaminide as substrate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.50 4°C, 10 mM sodium phosphate buffer, pH 7.2, 150 mM NaCl, 1 year, no significant loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O involved in the degradation of heparin and heparan sulfate Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?
3.2.1.50 methylumbelliferyl-N-acetyl-alpha-D-glucosaminide + H2O
-
Homo sapiens methylumbelliferone + N-acetyl-alpha-D-glucosamine
-
?
3.2.1.50 uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine
-
Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.50 dimer 2 * 90000, monomer of 90000 and dimer in a ratio 1:2, gel filtration Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 50 70 complete loss of activity by heating at 70°C for 4 min Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.50 4.6 8.1
-
Homo sapiens