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Literature summary extracted from

  • Srivastava, R.A.K.
    Purification and chemical characterization of thermostable amylases produced by Bacillus stearothermophilus (1987), Enzyme Microb. Technol., 9, 749-754.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 Ag+
-
Geobacillus stearothermophilus
3.2.1.2 Al3+ mild inhibitor Geobacillus stearothermophilus
3.2.1.2 Cd2+
-
Geobacillus stearothermophilus
3.2.1.2 EDTA non-competitive Geobacillus stearothermophilus
3.2.1.2 Fe3+
-
Geobacillus stearothermophilus
3.2.1.2 Hg2+
-
Geobacillus stearothermophilus
3.2.1.2 iodoacetic acid
-
Geobacillus stearothermophilus
3.2.1.2 Mg2+ mild inhibitor Geobacillus stearothermophilus
3.2.1.2 Mn2+ mild inhibitor Geobacillus stearothermophilus
3.2.1.2 Ni2+
-
Geobacillus stearothermophilus
3.2.1.2 Pb2+
-
Geobacillus stearothermophilus
3.2.1.2 PCMB
-
Geobacillus stearothermophilus
3.2.1.2 Zn2+ mild inhibitor Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 additional information
-
additional information Km: 1.25 mg/ml for starch Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.2 Ba2+ stimulates Geobacillus stearothermophilus
3.2.1.2 Ca2+ stimulates Geobacillus stearothermophilus
3.2.1.2 K+ stimulates Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 57000
-
gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.2 additional information
-
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 80
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.2 85 90 85°C: 83% of maximal activity, 90°C: 32.8% of maximal activity Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 6.9
-
-
Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.2 5.5 10 pH 5.5.: about 50% of maximal activity, pH 10.0: about 40% of maximal activity Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.2 7 11.5 20°C, 24 h, stable. Sharp decrease in activity at pH 12.0 Geobacillus stearothermophilus