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Literature summary extracted from

  • French, D.
    beta-Amylases (1960), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 4, 345-368.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 Ag+
-
Hordeum vulgare
3.2.1.2 Ag+ 0.001-0.1 mM AgNO3 Ipomoea batatas
3.2.1.2 Cu2+
-
Hordeum vulgare
3.2.1.2 Cu2+ 1 mM CuSO4, 50% inhibition Ipomoea batatas
3.2.1.2 Cu2+
-
Secale cereale
3.2.1.2 Hg2+
-
Hordeum vulgare
3.2.1.2 Hg2+ 0.001-0.1 mM HgCl2 Ipomoea batatas
3.2.1.2 iodoacetic acid partial Glycine max
3.2.1.2 Iodosobenzoate
-
Ipomoea batatas
3.2.1.2 NaCN 0.1% Glycine max
3.2.1.2 NaNO2 partial Glycine max
3.2.1.2 PCMB
-
Glycine max
3.2.1.2 PCMB
-
Hordeum vulgare
3.2.1.2 PCMB 0.001-0.1 mM, inactivation is partly reversed by adding 10fold to 20fold excess of glutathione or 1,2-dithiopropanol Ipomoea batatas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 152000
-
calculation from sedimentation and diffusion data Ipomoea batatas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.2 additional information Triticum aestivum
-
?
-
?
3.2.1.2 additional information Hordeum vulgare germination markedly increases the beta-amylase ?
-
?
3.2.1.2 additional information Secale cereale germination markedly increases the beta-amylase ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Glycine max
-
-
-
3.2.1.2 Hordeum vulgare
-
-
-
3.2.1.2 Ipomoea batatas
-
-
-
3.2.1.2 Secale cereale
-
-
-
3.2.1.2 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2
-
Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 additional information
-
Triticum aestivum ?
-
?
3.2.1.2 additional information germination markedly increases the beta-amylase Hordeum vulgare ?
-
?
3.2.1.2 additional information germination markedly increases the beta-amylase Secale cereale ?
-
?
3.2.1.2 starch + H2O
-
Triticum aestivum maltose + ?
-
?
3.2.1.2 starch + H2O
-
Secale cereale maltose + ?
-
?
3.2.1.2 starch + H2O soluble Hordeum vulgare maltose + ?
-
?
3.2.1.2 starch + H2O soluble Glycine max maltose + ?
-
?
3.2.1.2 starch + H2O soluble Ipomoea batatas maltose + ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 50 55
-
Ipomoea batatas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.2 additional information
-
additional information
-
Ipomoea batatas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 4 5 acetate buffer Ipomoea batatas
3.2.1.2 5.3
-
-
Triticum aestivum
3.2.1.2 5.5 6 citrate buffer Ipomoea batatas
3.2.1.2 6
-
-
Glycine max
3.2.1.2 6
-
-
Ipomoea batatas

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.2 3 8 pH 3.0: about 35% of maximal activity, pH 8.0: about 70% of maximal activity Glycine max
3.2.1.2 3 8 pH 3.0: about 70% of maximal activity, pH 8.0: about 60% of maximal activity Triticum aestivum
3.2.1.2 3 8.5 pH 3.0: about 30% of maximal activity, pH 8.5: about 40% of maximal activity Triticum aestivum