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Literature summary extracted from

  • Tani, N.; Ohtsuru, M.; Hata, T.
    Purification and general characteristics of bacterial myrosinase produced by Enterobacter cloacae (1974), Agric. Biol. Chem., 38, 1623-1630.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 2-mercaptoethanol 1 mM, activates Enterobacter cloacae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 1-O-methyl-alpha-D-glucopyranose
-
 Enterobacter cloacae
3.2.1.147 amygdalin
-
 Enterobacter cloacae
3.2.1.147 arbutin
-
 Enterobacter cloacae
3.2.1.147 ascorbic acid 0.1 mM L-ascorbic acid, 88% inhibition Enterobacter cloacae
3.2.1.147 Ca2+ 1 mM, CaCl2, 14% inhibition Enterobacter cloacae
3.2.1.147 Co2+ 1 mM, CoCl2, 27% inhibition Enterobacter cloacae
3.2.1.147 Cu+ 1 mM, CuCl, 56% inhibition Enterobacter cloacae
3.2.1.147 Cu2+ 1 mM, CuCl2, 78% inhibition Enterobacter cloacae
3.2.1.147 delta-gluconolactone
-
 Enterobacter cloacae
3.2.1.147 EDTA
-
 Enterobacter cloacae
3.2.1.147 Fe2+ 1 mM, FeCl2, 68% inhibition Enterobacter cloacae
3.2.1.147 Fe3+ 1 mM, FeCl3, 18% inhibition Enterobacter cloacae
3.2.1.147 fructose
-
 Enterobacter cloacae
3.2.1.147 galactose
-
 Enterobacter cloacae
3.2.1.147 glucose
-
 Enterobacter cloacae
3.2.1.147 HgCl2 1 mM, HgCl2, 87% inhibition Enterobacter cloacae
3.2.1.147 maltose
-
 Enterobacter cloacae
3.2.1.147 mannose
-
 Enterobacter cloacae
3.2.1.147 methyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
3.2.1.147 Mg2+ 1 mM, MgCl2, 22% inhibition Enterobacter cloacae
3.2.1.147 Ni2+ 1 mM, NiCl2, 30% inhibition Enterobacter cloacae
3.2.1.147 p-nitrophenyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
3.2.1.147 phenyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
3.2.1.147 Salicin
-
 Enterobacter cloacae
3.2.1.147 sorbitol
-
 Enterobacter cloacae
3.2.1.147 Sr2+ 1 mM, SrCl2, 21% inhibition Enterobacter cloacae
3.2.1.147 sucrose
-
 Enterobacter cloacae
3.2.1.147 Zn2+ 1 mM, ZnCl2, 45% inhibition Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.37
-
 sinigrin
-
 Enterobacter cloacae
3.2.1.147 0.71
-
 p-nitrophenyl beta-D-glucopyranoside
-
 Enterobacter cloacae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.147 Li+ 1 mM LiCl2, activates Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 61000
-
 gel filtration Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Enterobacter cloacae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 11.5
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Enterobacter cloacae ?
-
 ?
3.2.1.147 sinigrin + H2O
-
 Enterobacter cloacae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.147 30
-
 pH 6.8, 30 min, stable Enterobacter cloacae
3.2.1.147 40
-
 pH 6.8, 30 min, about 20% loss of activity Enterobacter cloacae
3.2.1.147 50
-
 pH 6.8, 30 min, about 50% loss of activity Enterobacter cloacae
3.2.1.147 60
-
 pH 6.8, 30 min, about 90% loss of activity Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 6.8
-
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.147 5.5 7.5 pH 5.5: about 40% of maximal activity, pH 7.5: about 60% of maximal activity Enterobacter cloacae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.147 5 7 below 40°C, 24 h, stable Enterobacter cloacae