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Literature summary extracted from

  • Tsuruo, I.; Hata, T.
    Studies on the myrosinase in mustard seed. Part III. On the effect of neutral salts (1968), Agric. Biol. Chem., 32, 479-483.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 KNO3 activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 NaBr activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 NaNO3 activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.094
-
 sinigrin in presence of 0.05 M NaCl Brassica juncea
3.2.1.147 0.18
-
 sinigrin in presence of 0.5 M NaCl Brassica juncea
3.2.1.147 0.26
-
 sinigrin in presence of 0.05 M NaCl and 1 mM ascorbate Brassica juncea
3.2.1.147 0.64
-
 sinigrin in presence of 2.5 M NaCl Brassica juncea
3.2.1.147 0.92
-
 sinigrin in presence of 0.5 M NaCl and 1 mM ascorbate Brassica juncea
3.2.1.147 2.78
-
 sinigrin in presence of 2.5 M NaCl and 1 mM ascorbate Brassica juncea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.147 (NH4)2SO4 activation is stronger in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 Ba2+ activation is stronger in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 Ca2+ slight activation in presence of 1 mM ascorbate, no effect in absence of ascorbate Brassica juncea
3.2.1.147 K+ K2SO4, activation is stronger in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 KNO3 activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 Mg2+ activation is stronger in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 Na+ Na2SO4, activation is stronger in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 NaBr activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea
3.2.1.147 NaNO3 activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate Brassica juncea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica juncea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 seed
-
 Brassica juncea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 sinigrin + H2O
-
 Brassica juncea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?