Any feedback?
Literature summary extracted from
- The interaction of L-ascorbic acid with the active center of myrosinase (1979), Biochim. Biophys. Acta, 567, 384-391.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.147 | ascorbic acid | activates, enzyme is conformationally changed, no activation by analogs | Brassica juncea |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.147 | 2-methoxy-5-nitrotropone | 1 mM, strong | Brassica juncea | |
| 3.2.1.147 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Brassica juncea | |
| 3.2.1.147 | p-mercuribenzoate | 0.1 mM, strong inhibition | Brassica juncea | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.147 | Brassica juncea | - |
i.e. yellow mustard | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.147 | sinigrin + H2O | - |
Brassica juncea | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 35 | - |
in presence of L-ascorbic acid | Brassica juncea |
| 3.2.1.147 | 55 | - |
in absence of L-ascorbic acid | Brassica juncea |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 25 | 50 | about 45% of maximal activity at 25°C and at 50°C, in presence of 1 mM ascorbic acid | Brassica juncea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
