Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ohtsuru, M.; Kawatani, H.
    Studies on the myrosinase from Wasabia japonica: purification and some properties of Wasabi myrosinase (1979), Agric. Biol. Chem., 43, 2249-2255.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid active only in presence of L-ascorbic acid, maximal activation at 2 mM Eutrema japonicum
3.2.1.147 ascorbyl palmitate lower activation than with ascorbic acid Eutrema japonicum
3.2.1.147 ascorbyl stearate lower activation than with ascorbic acid Eutrema japonicum
3.2.1.147 D-araboascorbate lower activation than with ascorbic acid Eutrema japonicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 1,10-phenanthroline
-
 Eutrema japonicum
3.2.1.147 2-methoxy-5-nitrotropone
-
 Eutrema japonicum
3.2.1.147 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Eutrema japonicum
3.2.1.147 Cu2+ 1 mM CuCl2, complete inhibition Eutrema japonicum
3.2.1.147 EDTA
-
 Eutrema japonicum
3.2.1.147 Fe3+ 1 mM FeCl3, 16% inhibition Eutrema japonicum
3.2.1.147 HgCl2 1 mM HgCl2, 93% inhibition Eutrema japonicum
3.2.1.147 p-mercuribenzoate
-
 Eutrema japonicum
3.2.1.147 Trinitrobenzenesulfonic acid
-
 Eutrema japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.47
-
 sinigrin
-
 Eutrema japonicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.147 Ca2+ 1 mM CaCl2, activates Eutrema japonicum
3.2.1.147 Co2+ 1 mM CoCl2, activates Eutrema japonicum
3.2.1.147 K+ 1 mM KCl2, activates Eutrema japonicum
3.2.1.147 Mg2+ 1 mM MgCl2, slight activation Eutrema japonicum
3.2.1.147 Mn2+ 1 mM MnCl2, activates Eutrema japonicum
3.2.1.147 Na+ 1 mM NaCl2, slight activation Eutrema japonicum
3.2.1.147 Ni2+ 1 mM NiCl2, activates Eutrema japonicum
3.2.1.147 Sn2+ 1 mM SnCl2, activates Eutrema japonicum
3.2.1.147 Sr2+ 1 mM SrCl2, activates Eutrema japonicum
3.2.1.147 Zn2+ 1 mM ZnCl2, activates Eutrema japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 580000
-
 gel filtration Eutrema japonicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Eutrema japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147
-
 Eutrema japonicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 additional information
-
-
 Eutrema japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 sinigrin + H2O
-
 Eutrema japonicum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 dodecamer 12 * 45000-47000, SDS-PAGE Eutrema japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 37
-
-
 Eutrema japonicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.147 30 50 about 40% of maximal activity at 30°C and at 50°C Eutrema japonicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.147 30
-
 pH 7.0, 20 min, stable Eutrema japonicum
3.2.1.147 40
-
 pH 7.0, 20 min, about 25% loss of activity Eutrema japonicum
3.2.1.147 50
-
 pH 7.0, 20 min, about 60% loss of activity Eutrema japonicum
3.2.1.147 70
-
 pH 7.0, 20 min, about 80% loss of activity Eutrema japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 6.5 7
-
 Eutrema japonicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.147 4 9 pH 4.0: about 45% of maximal activity, pH 9.0: about 40% of maximal activity Eutrema japonicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.147 4
-
 5°C, 41 h, about 60% loss of activity Eutrema japonicum
3.2.1.147 6
-
 5°C, 41 h, about 10% loss of activity Eutrema japonicum
3.2.1.147 7
-
 5°C, 41 h, stable Eutrema japonicum
3.2.1.147 10
-
 5°C, 41 h, about 25% loss of activity Eutrema japonicum
3.2.1.147 11
-
 5°C, 41 h, about 30% loss of activity Eutrema japonicum