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Literature summary extracted from

  • Rietschel-Berst, M.; Jentoft, N.H.; Rick, P.D.; Pletcher, C.; Fang, F.; Gander, J.E.
    Extracellular exo-beta-galactofuranosidase from Penicillium charlesii (1977), J. Biol. Chem., 252, 3219-3226.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.146 peptidophosphogalactomannan + H2O Penicillium charlesii
-
 peptidophosphogalactomannan + D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.146 Penicillium charlesii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.146 affinity chromatography on Sepharose 4B-peptidophosphogalactomannan Penicillium charlesii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.146 6.77
-
 with 70% galactose in substrate peptidophosphogalactomannan Penicillium charlesii

Storage Stability

EC Number Storage Stability Organism
3.2.1.146 25°C, 66 mM acetate buffer pH 4, 1 week, 20% loss in activity Penicillium charlesii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.146 methyl-beta-D-galactofuranoside + H2O
-
 Penicillium charlesii methanol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Penicillium charlesii p-nitrophenol + D-galactose
-
 ?
3.2.1.146 peptidophosphogalactomannan + H2O
-
 Penicillium charlesii peptidophosphogalactomannan + D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.146 47
-
-
 Penicillium charlesii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.146 3 4 sharp drop above pH 5, no activity above pH 7 Penicillium charlesii