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Literature summary extracted from

  • Tanuma, S.i.; Kawashima, K.; Endo, H.
    Purification and properties of an (ADP-ribose)n glycohydrolase from guinea pig liver nuclei (1986), J. Biol. Chem., 261, 965-969.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.143 2-mercaptoethanol 10 mM, 3fold stimulation Cavia porcellus
3.2.1.143 dithiothreitol 10 mM, 3fold stimulation Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.143 (17-chloro-3b-hydroxy-androsta-5,16-diene)-succinyl-glutathione
-
 Cavia porcellus
3.2.1.143 adenosine 3':5'-cyclic monophosphate
-
 Cavia porcellus
3.2.1.143 ADP-ribose
-
 Cavia porcellus
3.2.1.143 CaCl2 1.5 mM, 50% inhibition. 96% inhibition at 10 mM Cavia porcellus
3.2.1.143 DNA denatured; no inhibition by native DNA Cavia porcellus
3.2.1.143 histone
-
 Cavia porcellus
3.2.1.143 KCl 200 mM or more Cavia porcellus
3.2.1.143 NaCl 200 mM or more Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.143 0.0023
-
 (ADP-ribose)n
-
 Cavia porcellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.143 KCl 50 mM, 1.4-1.5fold stimulation Cavia porcellus
3.2.1.143 NaCl 50 mM, 1.4-1.5fold stimulation Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.143 72000
-
 gel filtration Cavia porcellus
3.2.1.143 75500
-
 1 * 75500, SDS-PAGE Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.143
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.143 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.143 32.5
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 poly(ADP-ribose)n + H2O
-
 Cavia porcellus ADP-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.143 monomer 1 * 75500, SDS-PAGE Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.143 6.8 7
-
 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.143 6 8 about 55% of maximal activity at pH 6.0 and 8.0 Cavia porcellus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.143 1.5
-
 CaCl2
-
 Cavia porcellus