EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.143 | 2-mercaptoethanol | 10 mM, required for maximal activity | Bos taurus | |
3.2.1.143 | dithiothreitol | 10 mM, required for maximal activity | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.143 | adenosine 3':5'-cyclic monophosphate | 0.3 mM, 50% inhibition | Bos taurus | |
3.2.1.143 | adenosine cyclic 2':3'-monophosphate | 10 mM, 23% inhibition | Bos taurus | |
3.2.1.143 | ADP | 10 mM, 39% inhibition | Bos taurus | |
3.2.1.143 | ADP-ribose | 1.1 mM, 50% inhibition | Bos taurus | |
3.2.1.143 | AMP | 10 mM, 64% inhibition | Bos taurus | |
3.2.1.143 | ATP | 10 mM, 23% inhibition | Bos taurus | |
3.2.1.143 | guanosine cyclic 3':5'-monophosphate | 10 mM, 42% inhibition | Bos taurus | |
3.2.1.143 | HgCl2 | - |
Bos taurus | |
3.2.1.143 | histone | histone f2a, f2b and f3. Inhibition by histone f2a is reversed by DNA | Bos taurus | |
3.2.1.143 | N6,O2'-dibutyryl adenosine cyclic 3':5'-monophosphate | 10 mM, 11% inhibition | Bos taurus | |
3.2.1.143 | N6-monobutyryl adenosine cyclic 3':5'-monophosphate | 10 mM, 50% inhibition | Bos taurus | |
3.2.1.143 | NAD+ | 10 mM, 40% inhibition | Bos taurus | |
3.2.1.143 | p-chloromercuribenzenesulfonate | 0.01 mM | Bos taurus | |
3.2.1.143 | poly(L-Lys) | - |
Bos taurus | |
3.2.1.143 | protamine | - |
Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.143 | 0.00058 | - |
(ADP-ribose)n | - |
Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.143 | 48000 | - |
gel filtration | Bos taurus |
3.2.1.143 | 53000 | - |
sucrose density gradient centrifugation | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.143 | poly(ADP-ribose)n + H2O | Bos taurus | the enzyme may regulate functionally the chain length of poly(ADP-ribose) which plays a role in DNA synthesis or in the structure of chromatin | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.143 | Bos taurus | - |
calf | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.143 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.143 | thymus | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.143 | 0.182 | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.143 | -80°C, stable for 3 months | Bos taurus |
3.2.1.143 | 4°C, 20% loss of activity after 1 month, addition of 0.1 mg/ml bovine serum albumin or 0.1 M NaCl to the diluted enzyme solution stabilizes the enzyme activity | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.143 | poly(ADP-ribose)n + H2O | - |
Bos taurus | ADP-ribose | - |
? | |
3.2.1.143 | poly(ADP-ribose)n + H2O | the enzyme may regulate functionally the chain length of poly(ADP-ribose) which plays a role in DNA synthesis or in the structure of chromatin | Bos taurus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.143 | 7.5 | - |
activity is less in Tris-HCl buffer than in sodium phosphate buffer or N-2-hydroxyethylpiperazine-N-ethanesulfonic acid-NaOH buffer | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.143 | 0.3 | - |
adenosine 3':5'-cyclic monophosphate | - |
Bos taurus | |
3.2.1.143 | 1.1 | - |
ADP-ribose | - |
Bos taurus | |
3.2.1.143 | 10 | - |
N6-monobutyryl adenosine cyclic 3':5'-monophosphate | - |
Bos taurus |