BRENDA - Enzyme Database

Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids

Kalinowska, M.; Wojciechowski, Z.A.; Phytochemistry 25, 45-49 (1986)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.173
Phospholipids
not stimulatory
Sinapis alba
2.4.1.173
Triton X-100
activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents
Sinapis alba
2.4.1.173
Tween-20
2fold activity stimulation at 0.1%
Sinapis alba
2.4.1.173
Tween-40
70% activity stimulation at 0.05%
Sinapis alba
2.4.1.173
Tween-60
35% activity stimulation at 0.1%
Sinapis alba
3.2.1.104
Cholesteryl acetate
150% stimulation at 0.1 mM
Sinapis alba
3.2.1.104
methyl stearate
150% stimulation at 0.1 mM
Sinapis alba
3.2.1.104
phytol
200% stimulation at 0.002 mM
Sinapis alba
3.2.1.104
Tripalmitoylglycerol
145% stimulation at 0.1 mM
Sinapis alba
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.173
sodium deoxycholate
strong inhibition at 0.1-1%
Sinapis alba
2.4.1.173
sodium taurocholate
strong inhibition at 0.1-1%
Sinapis alba
3.2.1.104
2-lyso-phosphatidylcholine
-
Sinapis alba
3.2.1.104
2-lyso-phosphatidylethanolamine
-
Sinapis alba
3.2.1.104
monogalactosyldiacylglycerol
-
Sinapis alba
3.2.1.104
phosphatidylcholine
-
Sinapis alba
3.2.1.104
phosphatidylethanolamine
-
Sinapis alba
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.173
membrane
organelles membrane
Sinapis alba
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.173
Sinapis alba
-
white mustard
-
3.2.1.104
Sinapis alba
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.173
partial purification by differential centrifugation and precipitation with Me2CO
Sinapis alba
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.173
seedling
-
Sinapis alba
-
3.2.1.104
seedling
-
Sinapis alba
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.173
-20 C, Tris-HCl 0.1 M, pH 7.2, two weeks
Sinapis alba
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.173
UDP-glucose + cholesterol
-
208429
Sinapis alba
UDP + cholesterol 3-beta-D-glucoside
-
208429
Sinapis alba
?
3.2.1.104
cholesteryl beta-D-glucoside + H2O
-
208429
Sinapis alba
D-glucose + cholesterol
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.173
30
-
assay at
Sinapis alba
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.1.173
7.2
-
Tris-HCl 0.1 M
Sinapis alba
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.173
Phospholipids
not stimulatory
Sinapis alba
2.4.1.173
Triton X-100
activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents
Sinapis alba
2.4.1.173
Tween-20
2fold activity stimulation at 0.1%
Sinapis alba
2.4.1.173
Tween-40
70% activity stimulation at 0.05%
Sinapis alba
2.4.1.173
Tween-60
35% activity stimulation at 0.1%
Sinapis alba
3.2.1.104
Cholesteryl acetate
150% stimulation at 0.1 mM
Sinapis alba
3.2.1.104
methyl stearate
150% stimulation at 0.1 mM
Sinapis alba
3.2.1.104
phytol
200% stimulation at 0.002 mM
Sinapis alba
3.2.1.104
Tripalmitoylglycerol
145% stimulation at 0.1 mM
Sinapis alba
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.173
sodium deoxycholate
strong inhibition at 0.1-1%
Sinapis alba
2.4.1.173
sodium taurocholate
strong inhibition at 0.1-1%
Sinapis alba
3.2.1.104
2-lyso-phosphatidylcholine
-
Sinapis alba
3.2.1.104
2-lyso-phosphatidylethanolamine
-
Sinapis alba
3.2.1.104
monogalactosyldiacylglycerol
-
Sinapis alba
3.2.1.104
phosphatidylcholine
-
Sinapis alba
3.2.1.104
phosphatidylethanolamine
-
Sinapis alba
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.173
membrane
organelles membrane
Sinapis alba
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.173
partial purification by differential centrifugation and precipitation with Me2CO
Sinapis alba
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.173
seedling
-
Sinapis alba
-
3.2.1.104
seedling
-
Sinapis alba
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.173
-20 C, Tris-HCl 0.1 M, pH 7.2, two weeks
Sinapis alba
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.173
UDP-glucose + cholesterol
-
208429
Sinapis alba
UDP + cholesterol 3-beta-D-glucoside
-
208429
Sinapis alba
?
3.2.1.104
cholesteryl beta-D-glucoside + H2O
-
208429
Sinapis alba
D-glucose + cholesterol
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.173
30
-
assay at
Sinapis alba
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.1.173
7.2
-
Tris-HCl 0.1 M
Sinapis alba