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Literature summary extracted from

  • Nakagawa, H.; Yamada, T.; Chien, J.L.; Gardas, A.; Kitamikado, M.; Li, S.C.; Li, Y.T.
    Isolation and characterization of an endo-beta-galactosidase from a new strain of Escherichia freundii (1980), J. Biol. Chem., 255, 5955-5959.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.103 analysis useful for studying the structure of glycoproteins and oligosaccharides Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.103 Ag+ 1 mM, complete inhibition Citrobacter freundii
3.2.1.103 Cu2+ 1 mM, 30% inhibition Citrobacter freundii
3.2.1.103 Hg2+ 1 mM, complete inhibition Citrobacter freundii
3.2.1.103 PCMB 0.25 mM, complete inhibition Citrobacter freundii
3.2.1.103 Zn2+ 1 mM, 30% inhibition Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.103 28000
-
 gel filtration Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.103 Citrobacter freundii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.103
-
 Citrobacter freundii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.103 culture medium
-
 Citrobacter freundii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.103 additional information
-
-
 Citrobacter freundii

Storage Stability

EC Number Storage Stability Organism
3.2.1.103 0°C, stable for several months Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.103 Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
 Citrobacter freundii Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
-
 ?
3.2.1.103 Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
 Citrobacter freundii ?
-
 ?
3.2.1.103 Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
 Citrobacter freundii Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer
-
 ?
3.2.1.103 Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
-
 Citrobacter freundii Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
-
 ?
3.2.1.103 keratan sulfate + H2O
-
 Citrobacter freundii keratan oligosaccharides 6-O-sulfo-GlcNAcbeta1-3Gal is the major product ?
3.2.1.103 NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
 Citrobacter freundii NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.103 37
-
 1 h, 50% loss of activity, bovine serum albumin, 0.4 mg/ml, prevents inactivation Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.103 5.5 5.8 hydrolysis of keratan sulfate from nasal cartilage or Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer Citrobacter freundii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.103 4
-
 17 h at room temperature, rapid loss of activity below Citrobacter freundii
3.2.1.103 4.5 5.5 17 h at room temperature, retains 80 to 100% activity Citrobacter freundii
3.2.1.103 6
-
 17 h at room temperature, rapid loss of activity above Citrobacter freundii