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Literature summary extracted from

  • Takasaki, S.; Kobata, A.
    Purification and characterization of an endo-beta-galactosidase produced by Diplococcus pneumoniae (1976), J. Biol. Chem., 251, 3603-3609.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.102 Ag2SO4 3 mM, 82% inhibition Streptococcus pneumoniae
3.2.1.102 Cys 3.3 mM, 15 min at 37°C, 52% inhibition Streptococcus pneumoniae
3.2.1.102 HgCl2 3 mM, 98.6% inhibition Streptococcus pneumoniae
3.2.1.102 PCMB 3.3 mM, 15 min at 37°C, 88% inhibition Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.102 0.068
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
3.2.1.102 0.13
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc
-
 Streptococcus pneumoniae
3.2.1.102 0.36
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
3.2.1.102 0.67
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.102 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.102
-
 Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.102 0.162
-
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.102 blood group substance A + H2O degradation of blood type A determinant Streptococcus pneumoniae GalNAcalpha1-3(Fucalpha1-2)Gal + Galalpha1-3(Fucalpha1-2)Gal + ?
-
 ?
3.2.1.102 blood group substance B + H2O degradation of blood type B determinant Streptococcus pneumoniae ?
-
 ?
3.2.1.102 Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.102 6
-
-
 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.102 5 7.5 pH 5.0: about 20% of maximal activity, pH 7.5: about 25% of maximal activity Streptococcus pneumoniae