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Literature summary extracted from

  • Kobata, A.; Takasaki, S.
    endo-beta-Galactosidase and endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae (1978), Methods Enzymol., 50, 560-584.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.97 analysis the enzyme is useful in the study of the structures of the carbohydrate moieties of various complex carbohydrates Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.97 EDTA 2.5 mM, 63% inhibition Streptococcus pneumoniae
3.2.1.97 Mn2+ 2.5 mM, 44% inhibition Streptococcus pneumoniae
3.2.1.97 p-chloromercuribenzenesulfonate 1 mM, 60% inhibition Streptococcus pneumoniae
3.2.1.97 Zn2+ 2.5 mM, 66% inhibition Streptococcus pneumoniae
3.2.1.102 Ag2SO4 3 mM, 82% inhibition Streptococcus pneumoniae
3.2.1.102 Cys 3.3 mM, 15 min at 37°C, 52% inhibition Streptococcus pneumoniae
3.2.1.102 HgCl2 3 mM, 98.6% inhibition Streptococcus pneumoniae
3.2.1.102 PCMB 3.3 mM, 15 min at 37°C, 88% inhibition Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.97 additional information
-
 additional information
-
 Streptococcus pneumoniae
3.2.1.102 0.068
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
3.2.1.102 0.13
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc
-
 Streptococcus pneumoniae
3.2.1.102 0.36
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
3.2.1.102 0.67
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.97 Ca2+ at a level twice the EDTA concentration completely restores enzyme activity Streptococcus pneumoniae
3.2.1.97 Mg2+ at a level twice the EDTA concentration completely restores enzyme activity Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.97 Streptococcus pneumoniae
-
-
-
3.2.1.102 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.97
-
 Streptococcus pneumoniae
3.2.1.102
-
 Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.102 culture medium
-
 Streptococcus pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.97 11.5
-
-
 Streptococcus pneumoniae
3.2.1.102 0.162
-
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.97 glycopeptide + H2O release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages Streptococcus pneumoniae Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Streptococcus pneumoniae Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.102 Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.102 additional information acts specifically on the oligosaccharides with blood group A and B antigenic determinants composed of type 2 chains: the A determinant present in erythrocyte glycoproteins is completely hydrolyzed Streptococcus pneumoniae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.97 6
-
-
 Streptococcus pneumoniae
3.2.1.102 6
-
-
 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.97 5.5 7 about 50% of maximal activity at pH 5.5 and at pH 7.0 Streptococcus pneumoniae