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Literature summary extracted from

  • Yano, T.; Funakoshi, I.; Yamashina, I.
    Purification and properties of nucleotide pyrophosphatase from human placenta (1985), J. Biochem., 98, 1097-1107.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 AMP
-
 Rattus norvegicus
3.1.4.1 glycine liver, 0.1 M glycine, inhibition initially 20-30%, 90% inhibition obtained after 60 min preincubation Rattus norvegicus
3.6.1.9 2,3-dimercapto-1-propanol nearly complete inactivation at 3 mM Homo sapiens
3.6.1.9 2-mercaptoethanol 80% inhibition at 5 mM Homo sapiens
3.6.1.9 Cu2+ inhibition at 5 mM Homo sapiens
3.6.1.9 EDTA abolishes activity and makes enzyme unstable Homo sapiens
3.6.1.9 glycine
-
 Homo sapiens
3.6.1.9 Hg2+ inhibition at 5 mM Homo sapiens
3.6.1.9 Ni2+ inhibition at 5 mM Homo sapiens
3.6.1.9 Zn2+ inhibition at 5 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Mg2+
-
 Rattus norvegicus
3.6.1.9 Mg2+ 10% activation at 10 mM, 5 mM obligatory for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 130000
-
 4 * 130000, SDS-PAGE Homo sapiens
3.6.1.9 520000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 ADP + H2O Homo sapiens low activity AMP + phosphate
-
 ?
3.6.1.9 ATP + H2O Homo sapiens
-
 AMP + diphosphate
-
 ?
3.6.1.9 GDP + H2O Homo sapiens low activity GMP + phosphate
-
 ?
3.6.1.9 GDP-mannose + H2O Homo sapiens
-
 GMP + D-mannose-1-phosphate
-
 ?
3.6.1.9 NADH + H2O Homo sapiens
-
 NMNH + AMP
-
 ?
3.6.1.9 poly (ADP-ribose) + H2O Homo sapiens
-
 phosphoribosyladenosine 5'-phosphate + ?
-
 ?
3.6.1.9 polynucleotide + H2O Homo sapiens hardly degraded nucleoside 5'-phosphate + ?
-
 ?
3.6.1.9 UDP-alpha-D-galactose + H2O Homo sapiens
-
 UMP + alpha-D-galactose-1-phosphate
-
 ?
3.6.1.9 UDP-glucose + H2O Homo sapiens
-
 UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UDP-N-acetyl-alpha-D-galactosamine + H2O Homo sapiens
-
 N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
-
 ?
3.6.1.9 UDP-N-acetyl-alpha-D-glucosamine + H2O Homo sapiens
-
 UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Rattus norvegicus
-
-
-
3.6.1.9 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.9 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 liver Rattus norvegicus
3.6.1.9 to homogeneity, affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 liver
-
 Rattus norvegicus
-
3.6.1.9 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 75 179
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.4.1 adenosine 5'-phosphosulfate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.4.1 ATP + H2O
-
 Rattus norvegicus 5'-AMP + diphosphate
-
 ?
3.1.4.1 additional information broad substrate specificity Rattus norvegicus ?
-
 ?
3.1.4.1 NAD+ + H2O
-
 Rattus norvegicus 5'-AMP + NMN
-
 ?
3.1.4.1 oligonucleotides + H2O
-
 Rattus norvegicus oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Rattus norvegicus 5'-TMP + p-nitrophenol
-
 ?
3.6.1.9 2'-5'-ApU + H2O
-
 Homo sapiens adenosine + uridine 5'-phosphate
-
 ?
3.6.1.9 3'-5'-ApA + H2O
-
 Homo sapiens adenosine + adenosine 5'-phosphate
-
 ?
3.6.1.9 3'-5'-ApU + H2O
-
 Homo sapiens adenosine + uridine 5'-phosphate
-
 ?
3.6.1.9 3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Homo sapiens 3'-phosphoadenosine + phosphosulfate
-
 ?
3.6.1.9 adenosine 5'-phosphosulfate + H2O
-
 Homo sapiens adenosine + phosphosulfate
-
 ?
3.6.1.9 ADP + H2O low activity Homo sapiens AMP + phosphate
-
 ?
3.6.1.9 ATP + H2O
-
 Homo sapiens AMP + diphosphate
-
 ?
3.6.1.9 GDP + H2O low activity Homo sapiens GMP + phosphate
-
 ?
3.6.1.9 GDP-mannose + H2O
-
 Homo sapiens GMP + D-mannose-1-phosphate
-
 ?
3.6.1.9 NADH + H2O
-
 Homo sapiens NMNH + AMP
-
 ?
3.6.1.9 poly (ADP-ribose) + H2O
-
 Homo sapiens phosphoribosyladenosine 5'-phosphate + ?
-
 ?
3.6.1.9 polynucleotide + H2O hardly degraded Homo sapiens nucleoside 5'-phosphate + ?
-
 ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Homo sapiens thymidine 5'-monophosphate + p-nitrophenol
-
 ?
3.6.1.9 UDP-alpha-D-galactose + H2O
-
 Homo sapiens UMP + alpha-D-galactose-1-phosphate
-
 ?
3.6.1.9 UDP-glucose + H2O
-
 Homo sapiens UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UDP-N-acetyl-alpha-D-galactosamine + H2O
-
 Homo sapiens N-acetyl-alpha-D-galactosaminyl-1-phosphate + UMP
-
 ?
3.6.1.9 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Homo sapiens UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 tetramer 4 * 130000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.9 NPPase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9 10
-
 Rattus norvegicus
3.6.1.9 10.6
-
-
 Homo sapiens