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Literature summary extracted from

  • Udvardy, J.; Marre, E.; Farkas, G.L.
    Purification and properties of a phosphodiesterase from Avena leaf tissues (1970), Biochim. Biophys. Acta, 206, 392-403.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.1 much enzyme activity is lost upon dialysis of the preparation against destilled water Avena sativa
3.1.4.1 repeated freezing and thawing inactivate phosphodiesterase Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 ADP
-
 Avena sativa
3.1.4.1 ATP
-
 Avena sativa
3.1.4.1 CN-
-
 Avena sativa
3.1.4.1 Cu2+ slight inhibition Avena sativa
3.1.4.1 CuSO4
-
 Avena sativa
3.1.4.1 EDTA
-
 Avena sativa
3.1.4.1 FAD
-
 Avena sativa
3.1.4.1 Hg2+
-
 Avena sativa
3.1.4.1 HgCl2
-
 Avena sativa
3.1.4.1 KH2PO4 slight inhibition Avena sativa
3.1.4.1 Na3AsO4 slight inhibition Avena sativa
3.1.4.1 NAD+ competitive Avena sativa
3.1.4.1 NADP+
-
 Avena sativa
3.1.4.1 NaF slight inhibition Avena sativa
3.1.4.1 phosphate slight inhibition Avena sativa
3.1.4.1 Urea slight inhibition Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.4
-
 p-nitrophenylthymidine 5'-phosphate
-
 Avena sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Ca2+ activation Avena sativa
3.1.4.1 Co2+ activation Avena sativa
3.1.4.1 divalent cations activation Avena sativa
3.1.4.1 divalent cations required for activity Avena sativa
3.1.4.1 Mg2+ activation Avena sativa
3.1.4.1 Zn2+ activation Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Avena sativa
-
 oat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1
-
 Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 leaf
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 0.183
-
-
 Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 bis(p-nitrophenyl) phosphate + H2O
-
 Avena sativa ?
-
 ?
3.1.4.1 DNA + H2O denatured DNA. Native DNA is resistant Avena sativa DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 additional information no activity with 2',3'-cyclic nucleotides Avena sativa ?
-
 ?
3.1.4.1 additional information enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal Avena sativa ?
-
 ?
3.1.4.1 additional information broad substrate specificity Avena sativa ?
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Avena sativa 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 poly A + H2O
-
 Avena sativa 5'-AMP
-
 ?
3.1.4.1 poly C + H2O
-
 Avena sativa 5'-CMP
-
 ?
3.1.4.1 poly U + H2O
-
 Avena sativa 5'-UMP
-
 ?
3.1.4.1 poly(I) + H2O slowly Avena sativa 5'-IMP
-
 ?
3.1.4.1 polynucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Avena sativa polynucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 RNA + H2O highly polymerized RNA: hydrolyzed at medium speed, single-stranded RNA: slowly, tRNA: not a substrate Avena sativa RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 alkaline phosphodiesterase
-
 Avena sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 80
-
 0.1 M Tris-acetate buffer, pH 6.8, 5 min: only 30% loss of original activity, enzyme shows considerable heat stability Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9.3
-
-
 Avena sativa