EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.1 | 2-mercaptoethanol | - |
Hordeum vulgare | |
3.1.4.1 | 5'-deoxyribonucleotides | end-product inhibition | Hordeum vulgare | |
3.1.4.1 | 5'-nucleotides | end-product inhibition | Hordeum vulgare | |
3.1.4.1 | 5'-ribonucleotides | end-product inhibition | Hordeum vulgare | |
3.1.4.1 | Cu2+ | - |
Hordeum vulgare | |
3.1.4.1 | CuSO4 | - |
Hordeum vulgare | |
3.1.4.1 | EDTA | - |
Hordeum vulgare | |
3.1.4.1 | glutathione | - |
Hordeum vulgare | |
3.1.4.1 | KF | slight inhibition | Hordeum vulgare | |
3.1.4.1 | L-cysteine | - |
Hordeum vulgare | |
3.1.4.1 | Reducing agents | - |
Hordeum vulgare | |
3.1.4.1 | Zn2+ | - |
Hordeum vulgare | |
3.1.4.1 | ZnSO4 | slight inhibition | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.1 | 0.28 | - |
p-nitrophenylthymidine 5'-phosphate | - |
Hordeum vulgare |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.1 | (NH4)2SO4 | slight activation | Hordeum vulgare | |
3.1.4.1 | Ca2+ | slight activation | Hordeum vulgare | |
3.1.4.1 | divalent cations | required for activity | Hordeum vulgare | |
3.1.4.1 | K+ | slight activation | Hordeum vulgare | |
3.1.4.1 | KCl | slight activation | Hordeum vulgare | |
3.1.4.1 | Mg2+ | activation | Hordeum vulgare | |
3.1.4.1 | MgCl2 | activation | Hordeum vulgare | |
3.1.4.1 | Na+ | slight activation | Hordeum vulgare | |
3.1.4.1 | NaCl | slight activation | Hordeum vulgare | |
3.1.4.1 | NH4Cl | slight activation | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 52000 | - |
52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
3.1.4.1 | 100000 | - |
gel filtration and PAGE | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.1 | additional information | Hordeum vulgare | the physiological function of PDE in plant cell is presumably the degradation of nucleic acids | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.1 | Hordeum vulgare | - |
barley | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.1 | - |
Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.1 | root | rootlet | Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 51.8 | - |
- |
Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.1 | DNA + H2O | denatured DNA. Native DNA is resistant | Hordeum vulgare | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
3.1.4.1 | additional information | the physiological function of PDE in plant cell is presumably the degradation of nucleic acids | Hordeum vulgare | ? | - |
? | |
3.1.4.1 | oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Hordeum vulgare | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
3.1.4.1 | p-nitrophenylthymidine 5'-phosphate + H2O | - |
Hordeum vulgare | 5'-TMP + p-nitrophenol | - |
? | |
3.1.4.1 | polynucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Hordeum vulgare | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
3.1.4.1 | RNA + H2O | - |
Hordeum vulgare | RNA(n-1) + nucleoside 5'-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.1 | dimer | 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
3.1.4.1 | trimer | 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.1 | 5'-PDE | - |
Hordeum vulgare |
3.1.4.1 | PDE I | - |
Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 9.5 | - |
- |
Hordeum vulgare |