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Literature summary extracted from

  • Fujimoto, M.; Fujiyama, K.; Midorikawa, Y.; Fujishima, T.; Kuninaka, A.; Yoshino, H.
    Properties of 5'-phosphodiesterase from Aspergillus niger (1977), Agric. Biol. Chem., 41, 737-744.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.4.1 synthesis production of 5'-mononucleotides, degradation of 2',5'-dinucleotides in nuclease P1 digest of technical grade yeast RNA Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 terminal 3'-monophosphoryl group of substrates
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 additional information no requirement Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Aspergillus awamori
-
-
-
3.1.4.1 Aspergillus batatae
-
-
-
3.1.4.1 Aspergillus carbonarius
-
-
-
3.1.4.1 Aspergillus fuscus
-
-
-
3.1.4.1 Aspergillus inuii
-
-
-
3.1.4.1 Aspergillus niger
-
-
-
3.1.4.1 Aspergillus phoenicis
-
-
-
3.1.4.1 Aspergillus usamii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 partial, Aspergillus niger AHU 7117 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 additional information
-
-
 Aspergillus niger
3.1.4.1 additional information
-
-
 Aspergillus phoenicis
3.1.4.1 additional information
-
-
 Aspergillus awamori
3.1.4.1 additional information
-
-
 Aspergillus usamii
3.1.4.1 additional information
-
-
 Aspergillus carbonarius
3.1.4.1 additional information
-
-
 Aspergillus inuii
3.1.4.1 additional information
-
-
 Aspergillus fuscus
3.1.4.1 additional information
-
-
 Aspergillus batatae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
-
 Aspergillus niger nucleoside 5'-monophosphate + p-nitrophenol
-
 ?
3.1.4.1 ADP + H2O
-
 Aspergillus niger AMP + phosphate
-
 ?
3.1.4.1 CDP-choline + H2O
-
 Aspergillus niger ?
-
 ?
3.1.4.1 dinucleoside monophosphates + H2O 3'-5' linkage Aspergillus niger mononucleoside 5'-phosphate
-
 ?
3.1.4.1 dinucleoside monophosphates + H2O no specificity toward bases or sugars Aspergillus niger mononucleoside 5'-phosphate
-
 ?
3.1.4.1 dinucleoside monophosphates + H2O 2'-5' linkage Aspergillus niger mononucleoside 5'-phosphate
-
 ?
3.1.4.1 dinucleotides + H2O 3'-hydroxyl-terminated 3'-5'and 2'-5' linkage without specificity toward bases or sugars Aspergillus niger ?
-
 ?
3.1.4.1 additional information no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP Aspergillus niger ?
-
 ?
3.1.4.1 oligonucleotides + H2O
-
 Aspergillus niger oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Aspergillus niger 5'-TMP + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.1 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 60
-
 stable below Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 5
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.1 3.3 5.3 stable between Aspergillus niger