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Literature summary extracted from

  • Brucato, C.L.; Wong, S.S.
    Extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium funiculosum: General characteristics and active site studies (1991), Arch. Biochem. Biophys., 290, 497-502.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.75 freezing and thawing diminishes activity Talaromyces funiculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 1,2-epoxy-3-(p-nitrophenoxy)propane complete inactivation at 100 mM, slow inactivation process Talaromyces funiculosus
3.1.1.75 diazo-DL-norleucine methyl ester complete inhibition at 25 mM, pseudo-first-order kinetic Talaromyces funiculosus
3.1.1.75 diisopropyl fluorophosphate complete inhibition at 500 nM Talaromyces funiculosus
3.1.1.75 dithiothreitol complete inhibition at 5 mM, reversible by H2O2 Talaromyces funiculosus
3.1.1.75 Hg2+ 90% inhibition at 6 mM, completely reversible by 2-mercaptoethanol Talaromyces funiculosus
3.1.1.75 Triton X-100 competitive inhibition, Ki: 0.014% Talaromyces funiculosus
3.1.1.75 Tween 80 competitive inhibition, Ki: 0.56% Talaromyces funiculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.75 additional information
-
 poly(3-hydroxybutanoate)n 0.17 mg/ml Talaromyces funiculosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Talaromyces funiculosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 37000
-
 gel filtration Talaromyces funiculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Talaromyces funiculosus
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Talaromyces funiculosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.75 glycoprotein 12% carbohydrate content by weight Talaromyces funiculosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 to homogeneity, single chromatography step Talaromyces funiculosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Talaromyces funiculosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.75 additional information
-
 1 unit defined as the amount of protein required to decrease the value of A650 by 1.0 per minute Talaromyces funiculosus
3.1.1.75 additional information
-
 51.2 U/mg Talaromyces funiculosus

Storage Stability

EC Number Storage Stability Organism
3.1.1.75 4°C, 10 mM sodium acetate, pH 6.0, 4 weeks Talaromyces funiculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyric acid) + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Talaromyces funiculosus poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.75 monomer 1 * 37000, SDS-PAGE Talaromyces funiculosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 40
-
 stable below for 15 min Talaromyces funiculosus
3.1.1.75 50
-
 completely inactive above Talaromyces funiculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 6
-
-
 Talaromyces funiculosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.75 1
-
 inactivation below Talaromyces funiculosus
3.1.1.75 10
-
 inactivation above Talaromyces funiculosus