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Literature summary extracted from
- Isolation of poly(3-hydroxybutyrate) (PHB)-degrading microorganisms and characterization of PHB-depolymerase from Arthrobacter sp. strain W6 (2001), Biosci. Biotechnol. Biochem., 65, 1191-1194.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | degradation | development of biodegradable plastics such as poly(3-hydroxybutyrate) or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Arthrobacter sp. |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.75 | unstable to (NH4)2SO4 treatment | Arthrobacter sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.75 | Ag+ | marked inhibition at 0.1 mM | Arthrobacter sp. |  |
| 3.1.1.75 | Hg2+ | marked inhibition at 0.1 mM | Arthrobacter sp. | |
| 3.1.1.75 | Pb2+ | marked inhibition at 0.1 mM | Arthrobacter sp. | |
| 3.1.1.75 | PMSF | marked inhibition at 0.1 mM | Arthrobacter sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.75 | extracellular | - |
Arthrobacter sp. | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 47000 | - |
native PAGE, SDS-PAGE | Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Arthrobacter sp. | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.75 | Arthrobacter sp. | - |
strains W1 and W5, degradation of BIOPOL only, strain W6, degradation of poly[(R)-3-hydroxybutanoate]n, BIOPOL and other polyhydroxyalkanoic acids | - |
| 3.1.1.75 | Pseudomonas alcaligenes | - |
strains W2, W3 and W4, degradation of BIOPOL | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.75 | to homogeneity, chromatography steps | Arthrobacter sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.75 | cell culture | - |
Arthrobacter sp. | - |
| 3.1.1.75 | cell culture | - |
Pseudomonas alcaligenes | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | additional information | - |
32.1 mg poly[(R)-3-hydroxybutanoate] /min * mg protein | Arthrobacter sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.75 | 4°C, culture supernatant, stable up to 20 days | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O | BIOPOL | Arthrobacter sp. | poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O | BIOPOL | Pseudomonas alcaligenes | poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate)n + H2O | - |
Arthrobacter sp. | poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate) | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate)n + H2O | best carbon source for enzyme synthesis in strain W6 | Arthrobacter sp. | poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate) | - |
? | |
| 3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Arthrobacter sp. | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | monomer | 1 * 47000, SDS-PAGE | Arthrobacter sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 50 | - |
- |
Arthrobacter sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 40 | - |
stable below | Arthrobacter sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 8.5 | - |
- |
Arthrobacter sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 6 | 7 | more than 95% of maximal activity | Arthrobacter sp. |
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