EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.2 | Cyanate | stimulates | Acinetobacter calcoaceticus | |
1.7.7.2 | Cyanate | stimulates | Azotobacter chroococcum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | CO | enzyme from higher plants and algae | [Chlorella] fusca | |
1.7.7.1 | CO | enzyme from higher plants and algae | Cucurbita pepo | |
1.7.7.1 | CO | enzyme from higher plants and algae | Neopyropia yezoensis | |
1.7.7.1 | CO | enzyme from higher plants and algae | Spinacia oleracea | |
1.7.7.1 | cyanide | - |
[Chlorella] fusca | |
1.7.7.1 | cyanide | - |
Cucurbita pepo | |
1.7.7.1 | cyanide | - |
Neopyropia yezoensis | |
1.7.7.1 | cyanide | - |
Spinacia oleracea | |
1.7.7.1 | Mersalyl | - |
Cucurbita pepo | |
1.7.7.1 | p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | [Chlorella] fusca | |
1.7.7.1 | p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Cucurbita pepo | |
1.7.7.1 | p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Neopyropia yezoensis | |
1.7.7.1 | p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Spinacia oleracea | |
1.7.7.2 | cyanide | - |
Acinetobacter calcoaceticus | |
1.7.7.2 | cyanide | - |
Azotobacter chroococcum | |
1.7.7.2 | cyanide | powerful inhibitor | Synechococcus elongatus PCC 7942 = FACHB-805 | |
1.7.7.2 | p-hydroxymercuribenzoate | - |
Acinetobacter calcoaceticus | |
1.7.7.2 | p-hydroxymercuribenzoate | - |
Azotobacter chroococcum | |
1.7.7.2 | p-hydroxymercuribenzoate | powerful inhibitor | Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.2 | additional information | - |
additional information | - |
Rhodotorula glutinis | |
1.7.1.2 | additional information | - |
additional information | - |
Ankistrodesmus braunii | |
1.7.7.1 | additional information | - |
additional information | - |
Spinacia oleracea | |
1.7.7.1 | additional information | - |
additional information | - |
[Chlorella] fusca | |
1.7.7.1 | additional information | - |
additional information | - |
Cucurbita pepo | |
1.7.7.1 | additional information | - |
additional information | - |
Neopyropia yezoensis | |
1.7.7.2 | 0.013 | - |
reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
1.7.7.2 | 0.69 | - |
nitrate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.7.1 | particle-bound | chlorophyll-containing subcellular particle | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
1.7.7.1 | soluble | exception: Anacystis | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
1.7.7.2 | membrane | tightly bound to chlorophyll-containing membrane fractions | Synechococcus elongatus PCC 7942 = FACHB-805 | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | Iron | - |
[Chlorella] fusca | |
1.7.7.1 | Iron | - |
Cucurbita pepo | |
1.7.7.1 | Iron | - |
Neopyropia yezoensis | |
1.7.7.1 | Iron | enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule | Spinacia oleracea | |
1.7.7.2 | Molybdenum | molybdoprotein | Synechococcus elongatus PCC 7942 = FACHB-805 | |
1.7.7.2 | Molybdenum | molybdoprotein | Acinetobacter calcoaceticus | |
1.7.7.2 | Molybdenum | molybdoprotein | Rhodobacter capsulatus | |
1.7.7.2 | Molybdenum | molybdoprotein | Azotobacter chroococcum | |
1.7.7.2 | Molybdenum | enzyme contains one molybdenum atom | Rhodobacter capsulatus | |
1.7.7.2 | Molybdenum | essential role in catalytic activity | Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 60000 | 63000 | gel filtration, sedimentation equilibrium | Spinacia oleracea |
1.7.7.1 | 63000 | - |
- |
Spinacia oleracea |
1.7.7.1 | 63000 | - |
- |
[Chlorella] fusca |
1.7.7.1 | 63000 | - |
- |
Cucurbita pepo |
1.7.7.1 | 63000 | - |
- |
Neopyropia yezoensis |
1.7.7.2 | 75000 | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
1.7.7.2 | 85000 | - |
2 * 85000 | Rhodobacter capsulatus |
1.7.7.2 | 100000 | - |
about | Acinetobacter calcoaceticus |
1.7.7.2 | 100000 | - |
about | Azotobacter chroococcum |
1.7.7.2 | 185000 | - |
- |
Rhodobacter capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.1 | nitrite + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Spinacia oleracea | - |
ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | [Chlorella] fusca | - |
ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Cucurbita pepo | - |
ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Neopyropia yezoensis | - |
ammonia + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Anabaena cylindrica | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Desmonostoc muscorum | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Anabaena cylindrica | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Desmonostoc muscorum | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced flavodoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation | nitrite + oxidized flavodoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.2 | Ankistrodesmus braunii | - |
- |
- |
1.7.1.2 | Rhodotorula glutinis | - |
- |
- |
1.7.7.1 | Cucurbita pepo | - |
- |
- |
1.7.7.1 | Neopyropia yezoensis | - |
- |
- |
1.7.7.1 | Spinacia oleracea | - |
- |
- |
1.7.7.1 | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
1.7.7.1 | [Chlorella] fusca | - |
- |
- |
1.7.7.2 | Acinetobacter calcoaceticus | - |
- |
- |
1.7.7.2 | Anabaena cylindrica | - |
- |
- |
1.7.7.2 | Azotobacter chroococcum | - |
- |
- |
1.7.7.2 | Desmonostoc muscorum | - |
- |
- |
1.7.7.2 | Rhodobacter capsulatus | - |
- |
- |
1.7.7.2 | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.7.2 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
1.7.7.2 | - |
Rhodobacter capsulatus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Synechococcus elongatus PCC 7942 = FACHB-805 | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Spinacia oleracea | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | [Chlorella] fusca | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Cucurbita pepo | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Neopyropia yezoensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.7.2 | additional information | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
1.7.7.2 | additional information | - |
- |
Rhodobacter capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.2 | NAD(P)H + nitrate | - |
Rhodotorula glutinis | NAD(P)+ + nitrite + H2O | - |
? | |
1.7.1.2 | NAD(P)H + nitrate | - |
Ankistrodesmus braunii | NAD(P)+ + nitrite + H2O | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
[Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donor: methyl viologen | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donor: methyl viologen | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donor: methyl viologen | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donor: methyl viologen | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donors: flavodoxin | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donors: flavodoxin | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donors: flavodoxin | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | other electron donors: flavodoxin | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | - |
Acinetobacter calcoaceticus | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | - |
Rhodobacter capsulatus | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | - |
Azotobacter chroococcum | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Anabaena cylindrica | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Desmonostoc muscorum | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | assimilatory nitrate reduction | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | assimilatory nitrate reduction | Anabaena cylindrica | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | assimilatory nitrate reduction | Desmonostoc muscorum | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Anabaena cylindrica | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Desmonostoc muscorum | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced flavodoxin | flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized flavodoxin + H2O | - |
? | |
1.7.7.2 | nitrate + reduced methyl viologen | enzyme uses also reduced methyl viologen as reductant | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized methyl viologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.7.1 | monomer | - |
Spinacia oleracea |
1.7.7.1 | monomer | - |
[Chlorella] fusca |
1.7.7.2 | dimer | 2 * 85000 | Rhodobacter capsulatus |
1.7.7.2 | monomer | 1 * 75000 | Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.7.1 | 10 | - |
nitrite | - |
Neopyropia yezoensis | |
1.7.7.1 | 54 | - |
nitrite | - |
[Chlorella] fusca | |
1.7.7.1 | 89 | - |
nitrite | - |
Cucurbita pepo | |
1.7.7.1 | 110 | - |
nitrite | - |
Spinacia oleracea | |
1.7.7.2 | 1090 | - |
nitrate | dithionite-reduced methyl viologen as electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.1.2 | 7.5 | - |
- |
Rhodotorula glutinis |
1.7.1.2 | 7.5 | - |
- |
Ankistrodesmus braunii |
1.7.7.1 | 7.5 | - |
- |
Spinacia oleracea |
1.7.7.1 | 7.5 | - |
- |
[Chlorella] fusca |
1.7.7.1 | 7.5 | - |
- |
Cucurbita pepo |
1.7.7.1 | 7.5 | - |
- |
Neopyropia yezoensis |
1.7.7.2 | 10.5 | - |
dithionite-reduced methyl viologen as electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.2 | cytochrome b557 | 1 mol per mol of enzyme | Rhodotorula glutinis | |
1.7.1.2 | cytochrome b557 | 4 mol per mol of enzyme | Ankistrodesmus braunii | |
1.7.7.1 | siroheme | prosthetic group | Spinacia oleracea | |
1.7.7.1 | siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea | |
1.7.7.2 | heme c | enzyme contains 1.5 heme molecules of the c-type | Rhodobacter capsulatus | |
1.7.7.2 | additional information | no presence of flavin, heme or cytochrome | Synechococcus elongatus PCC 7942 = FACHB-805 |