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Literature summary extracted from

  • De La Rosa, M.A.; Diez, J.; Vega, J.M.; Losada, M.
    Purification and properties of assimilatory nitrate reductase [NAD(P)H] from Ankistrodesmus braunii (1980), Eur. J. Biochem., 106, 249-256.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.2 p-hydroxymercuribenzoate
-
 Ankistrodesmus braunii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.2 460000 467400 Ankistrodesmus braunii, gel filtration, density gradient centrifugation, disc gel electrophoresis, pore gradient electrophoresis, sucrose-density gradient centrifugation Ankistrodesmus braunii

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.2 Ankistrodesmus braunii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.2
-
 Ankistrodesmus braunii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.2 61.25
-
-
 Ankistrodesmus braunii
1.7.1.2 72 80
-
 Ankistrodesmus braunii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.2 NAD(P)H + nitrate
-
 Ankistrodesmus braunii NAD(P)+ + nitrite + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.2 octamer 8 * 58750-59000, Ankistrodesmus braunii, SDS-PAGE Ankistrodesmus braunii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.2 470
-
 NADPH
-
 Ankistrodesmus braunii

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.2 heme minimum of 4 molecules of heme per molecule of native enzyme Ankistrodesmus braunii
1.7.1.2 NADH NADPH to NADH activity ratio is 1.8 Ankistrodesmus braunii
1.7.1.2 NADPH NADPH to NADH activity ratio is 1.8 Ankistrodesmus braunii