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Literature summary extracted from

  • Guerrero, M.G.; Gutierrez, M.
    Purification and properties of the NAD(P)H:nitrate reductase of the yeast Rhodotorula glutinis (1977), Biochim. Biophys. Acta, 482, 272-285.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.2 azide
-
 Rhodotorula glutinis
1.7.1.2 Cyanate
-
 Rhodotorula glutinis
1.7.1.2 cyanide
-
 Rhodotorula glutinis
1.7.1.2 nitrite
-
 Rhodotorula glutinis
1.7.1.2 p-chloromercuribenzoate
-
 Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.2 0.02
-
 NADPH
-
 Rhodotorula glutinis
1.7.1.2 0.125
-
 nitrate cosubstrate NADPH Rhodotorula glutinis
1.7.1.2 0.16
-
 NADH
-
 Rhodotorula glutinis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.2 Molybdenum molybdoprotein Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.2 118000
-
 2 * 118000, Rhodotorula glutinis, SDS-PAGE Rhodotorula glutinis
1.7.1.2 230000
-
 Rhodotorula glutinis, sucrose density gradient centrifugation, gel filtration Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.2 Rhodotorula glutinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.2
-
 Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.2 148.2
-
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.2 NAD(P)H + nitrate also reduction of ClO3-, K3Fe(CN)6, cytochrome c and 2,6-dichlorophenolindophenol with NADPH or NADH Rhodotorula glutinis NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.2 NAD(P)H + nitrate reduced methyl viologen Rhodotorula glutinis NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.2 NAD(P)H + nitrate other electron donors: combination of dithionite with flavin nucleotides or viologens Rhodotorula glutinis NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.2 nitrate + NADH + H+
-
 Rhodotorula glutinis nitrite + NAD+ + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.7.1.2 dimer 2 * 118000, Rhodotorula glutinis, SDS-PAGE Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.2 7.5
-
-
 Rhodotorula glutinis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.2 cytochrome b557 contains cytochrome b557 Rhodotorula glutinis
1.7.1.2 FAD complete reduction of cytochrome by NADPH takes place only when added FAD is present in the enzyme preparation before addition of NADPH Rhodotorula glutinis
1.7.1.2 NADH at low nitrate concentration: more active with NADH Rhodotorula glutinis
1.7.1.2 NADH at high nitrate concentration: more active with NADPH Rhodotorula glutinis
1.7.1.2 NADPH at low nitrate concentration: more active with NADH Rhodotorula glutinis
1.7.1.2 NADPH at high nitrate concentration: more active with NADPH Rhodotorula glutinis