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Literature summary extracted from

  • Saikusa, T.; Rhee, H.; Watanabe, K.; Murata, K.; Kimura, A.
    Metabolism of 2-oxoaldehydes in bacteria: purification and characterization of methylglyoxal reductase from Escherichia coli (1987), Agric. Biol. Chem., 51, 1893-1899.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.78 2-mercaptoethanol slight activation Escherichia coli
1.1.1.78 dithiothreitol slight activation Escherichia coli
1.1.1.78 glutathione slight activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.78 Co2+
-
 Escherichia coli
1.1.1.78 Cu2+
-
 Escherichia coli
1.1.1.78 Hg2+
-
 Escherichia coli
1.1.1.78 iodoacetate
-
 Escherichia coli
1.1.1.78 N-ethylmaleimide
-
 Escherichia coli
1.1.1.78 Ni2+
-
 Escherichia coli
1.1.1.78 p-chloromercuribenzoate
-
 Escherichia coli
1.1.1.78 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.78 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.78 43000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.78 methylglyoxal + NADH Escherichia coli
-
 (R)-lactaldehyde + NAD+
-
 ?
1.1.1.78 methylglyoxal + NADPH Escherichia coli
-
 lactaldehyde + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.78 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.78
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.78 2.26
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.78 methylglyoxal + NADH
-
 Escherichia coli (R)-lactaldehyde + NAD+
-
 ?
1.1.1.78 methylglyoxal + NADPH
-
 Escherichia coli lactaldehyde + NADP+
-
 ?
1.1.1.78 additional information enzyme utilizes both: NADH and NADPH Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.78 monomer 1 * 43000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.78 40
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.78 25 75 25°C and 75°C: about 50% of activity maximum Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.78 6.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.78 4.5 9.5 pH 4.5 and pH 9.5: about 50% of activity maximum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.78 NADH utilizes both NADPH and NADH Escherichia coli
1.1.1.78 NADPH utilizes both NADH and NADPH Escherichia coli