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Literature summary extracted from

  • Lim, Y.H.; Yokoigawa, K.; Esaki, N.; Soda, K.
    A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida: purification and characterization (1993), J. Bacteriol., 175, 4213-4217.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.10 0.42
-
D-Lys
-
Pseudomonas putida
5.1.1.10 0.55
-
L-Lys
-
Pseudomonas putida
5.1.1.10 9.8
-
D-Thr
-
Pseudomonas putida
5.1.1.10 31
-
L-Thr
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.6 41000
-
2 * 41000 Pseudomonas putida
5.1.1.6 82000
-
-
Pseudomonas putida
5.1.1.10 41000
-
2 * 41000, SDS-PAGE Pseudomonas putida
5.1.1.10 82000
-
gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.6 Pseudomonas putida
-
-
-
5.1.1.10 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.6
-
Pseudomonas putida
5.1.1.10
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.10 920
-
L-Lys as substrate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.6 L-Thr
-
Pseudomonas putida D-Thr
-
?
5.1.1.6 additional information the enzyme also racemizes various amino acids, except acidic and aromatic amino acids Pseudomonas putida ?
-
?
5.1.1.10 D-Arg 80% of the activity relative to L-Lys Pseudomonas putida L-Arg
-
?
5.1.1.10 D-Thr
-
Pseudomonas putida L-Thr
-
r
5.1.1.10 L-Ala 6% of the activity relative to L-Lys Pseudomonas putida D-Ala
-
?
5.1.1.10 L-Asn 12% of the activity relative to L-Lys Pseudomonas putida D-Asn
-
?
5.1.1.10 L-Citrulline 45% of the activity relative to L-Lys Pseudomonas putida D-Citrulline
-
?
5.1.1.10 L-Ethionine 83% of the activity relative to L-Lys Pseudomonas putida D-Ethionine
-
?
5.1.1.10 L-Glu 80% of the activity relative to L-Lys Pseudomonas putida D-Glu
-
?
5.1.1.10 L-His 6% of the activity relative to L-Lys Pseudomonas putida D-His
-
?
5.1.1.10 L-Homoarginine 80% of the activity relative to L-Lys Pseudomonas putida L-Homoarginine
-
?
5.1.1.10 L-Homocitrulline 45% of the activity relative to L-Lys Pseudomonas putida D-Homocitrulline
-
?
5.1.1.10 L-homoserine 22% of the activity relative to L-Lys Pseudomonas putida D-homoserine
-
?
5.1.1.10 L-Leu 10% of the activity relative to L-Lys Pseudomonas putida D-Leu
-
?
5.1.1.10 L-Lys
-
Pseudomonas putida D-Lys
-
?
5.1.1.10 L-Met 66% of the activity relative to L-Lys Pseudomonas putida D-Met
-
?
5.1.1.10 L-Norleucine 45% of the activity relative to L-Lys Pseudomonas putida D-Norleucine
-
?
5.1.1.10 L-Orn 80% of the activity relative to L-Lys Pseudomonas putida D-Orn
-
?
5.1.1.10 L-Ser 10% of the activity relative to L-Lys Pseudomonas putida D-Ser
-
?
5.1.1.10 L-Thr 3% of the activity relative to L-Lys Pseudomonas putida L-Thr
-
?
5.1.1.10 N6-Acetyl-L-Lys 80% of the activity relative to L-Lys Pseudomonas putida N6-Acetyl-D-Lys
-
?
5.1.1.10 S-Methyl-L-Cys 3% of the activity relative to L-Lys Pseudomonas putida S-Methyl-D-Cys
-
?

Subunits

EC Number Subunits Comment Organism
5.1.1.6 dimer 2 * 41000 Pseudomonas putida
5.1.1.10 dimer 2 * 41000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.6 pyridoxal 5'-phosphate contains 1 mol of pyridoxal 5'-phosphate per mol of subunit Pseudomonas putida
5.1.1.10 pyridoxal 5'-phosphate holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Pseudomonas putida
5.1.1.10 pyridoxal 5'-phosphate contains 1 mol of pyridoxal 5'-phosphate per mol of subunit Pseudomonas putida