EC Number | General Stability | Organism |
---|---|---|
6.5.1.4 | not affected by several cycles of freezing and thawing | Homo sapiens |
6.5.1.4 | resistant to protease digestion | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.4 | iodoacetamide | - |
Homo sapiens | |
6.5.1.4 | NEM | - |
Homo sapiens | |
6.5.1.4 | Vanadyl-ribonucleoside complex | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.5.1.4 | 0.006 | - |
ATP | - |
Homo sapiens | |
6.5.1.4 | 0.006 | - |
ATPgammaS | ATP | Homo sapiens | |
6.5.1.4 | 0.2 | - |
GTP | - |
Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.5.1.4 | nucleus | - |
Homo sapiens | 5634 | - |
6.5.1.4 | nucleus | - |
Xenopus laevis | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.4 | Ca2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
6.5.1.4 | K+ | optimal concentration: 150-200 mM | Homo sapiens | |
6.5.1.4 | Mg2+ | maximal activity at 4-5 mM | Homo sapiens | |
6.5.1.4 | Mn2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
6.5.1.4 | Na+ | optimal concentration: 150-200 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 38000 | 40000 | glycerol density gradient sedimentation, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.4 | additional information | Xenopus laevis | it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.4 | Homo sapiens | - |
- |
- |
6.5.1.4 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.4 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.5.1.4 | HeLa cell | - |
Homo sapiens | - |
6.5.1.4 | oocyte | - |
Xenopus laevis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | additional information | - |
- |
Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
6.5.1.4 | -70°C, stable for at least 6 months | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.4 | ATP + RNA 3'-terminal-phosphate | - |
Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | ATP + RNA 3'-terminal-phosphate | - |
Xenopus laevis | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | ATPgammaS + RNA 3'-terminal-phosphate | - |
Homo sapiens | AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | CTP + RNA 3'-terminal-phosphate | reaction proceeds 15-20 times slower than in presence of ATP | Homo sapiens | CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | GTP + RNA 3'-terminal-phosphate | reaction proceeds 15-20 times slower than in presence of ATP | Homo sapiens | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | additional information | it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends | Xenopus laevis | ? | - |
? | |
6.5.1.4 | UTP + RNA 3'-terminal-phosphate | reaction proceeds 15-20 times slower than in presence of ATP | Homo sapiens | UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.5.1.4 | monomer | 1 * 38000-40000, SDS-PAGE | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 30 | - |
similar rates of reaction at 25°C, 30°C, and 37°C | Homo sapiens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | - |
37 | 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 4 | - |
3 d, 50% loss of activity | Homo sapiens |
6.5.1.4 | 20 | - |
3-4 h, 50% loss of activity | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 8 | 9 | - |
Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 8 | 10.5 | 8.0-9.0: maximal activity, 10.5: 70% of maximal activity | Homo sapiens |