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Literature summary extracted from

  • Filipowicz, W.; Vicente, O.
    RNA 3'-terminal phosphate cyclase from HeLa cells (1990), Methods Enzymol., 181, 499-510.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.5.1.4 not affected by several cycles of freezing and thawing Homo sapiens
6.5.1.4 resistant to protease digestion Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.4 iodoacetamide
-
Homo sapiens
6.5.1.4 NEM
-
Homo sapiens
6.5.1.4 Vanadyl-ribonucleoside complex
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.4 0.006
-
ATP
-
Homo sapiens
6.5.1.4 0.006
-
ATPgammaS ATP Homo sapiens
6.5.1.4 0.2
-
GTP
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.5.1.4 nucleus
-
Homo sapiens 5634
-
6.5.1.4 nucleus
-
Xenopus laevis 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.4 Ca2+ only 5% of the activation by Mg2+ Homo sapiens
6.5.1.4 K+ optimal concentration: 150-200 mM Homo sapiens
6.5.1.4 Mg2+ maximal activity at 4-5 mM Homo sapiens
6.5.1.4 Mn2+ only 5% of the activation by Mg2+ Homo sapiens
6.5.1.4 Na+ optimal concentration: 150-200 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.4 38000 40000 glycerol density gradient sedimentation, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.4 additional information Xenopus laevis it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.4 Homo sapiens
-
-
-
6.5.1.4 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.4
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.4 HeLa cell
-
Homo sapiens
-
6.5.1.4 oocyte
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.4 additional information
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.5.1.4 -70°C, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
Xenopus laevis AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
6.5.1.4 ATPgammaS + RNA 3'-terminal-phosphate
-
Homo sapiens AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
6.5.1.4 CTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
6.5.1.4 GTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
6.5.1.4 additional information it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends Xenopus laevis ?
-
?
6.5.1.4 UTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
6.5.1.4 monomer 1 * 38000-40000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.4 30
-
similar rates of reaction at 25°C, 30°C, and 37°C Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.4
-
37 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.4 4
-
3 d, 50% loss of activity Homo sapiens
6.5.1.4 20
-
3-4 h, 50% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.4 8 9
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.4 8 10.5 8.0-9.0: maximal activity, 10.5: 70% of maximal activity Homo sapiens