EC Number | General Stability | Organism |
---|---|---|
6.5.1.4 | not affected by several cycles of freezing and thawing | Homo sapiens |
6.5.1.4 | resistant to protease digestion | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.4 | iodoacetamide | - |
Homo sapiens | |
6.5.1.4 | NEM | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.5.1.4 | 0.006 | - |
ATP | - |
Homo sapiens | |
6.5.1.4 | 0.006 | - |
ATPgammaS | ATP | Homo sapiens | |
6.5.1.4 | 0.2 | - |
GTP | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.4 | Ca2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
6.5.1.4 | K+ | optimal concentration: 150-200 mM | Homo sapiens | |
6.5.1.4 | Mg2+ | required | Homo sapiens | |
6.5.1.4 | Mg2+ | maximal activity at 4-5 mM | Homo sapiens | |
6.5.1.4 | Mn2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
6.5.1.4 | Na+ | optimal concentration: 150-200 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 38000 | 40000 | glycerol density gradient sedimentation, gel filtration | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.4 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.4 | - |
Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.5.1.4 | ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside | formation of a covalent cyclase-AMP intermediate | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.5.1.4 | HeLa cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | additional information | - |
- |
Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
6.5.1.4 | -70°C, stable for at least 6 months | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.4 | ATP + RNA 3'-terminal-phosphate | no activity with dATP | Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | ATPgammaS + RNA 3'-terminal-phosphate | - |
Homo sapiens | AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | CTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | GTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
6.5.1.4 | UTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.5.1.4 | monomer | 1 * 38000-40000, SDS-PAGE | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 30 | - |
similar rates of reaction at 25°C, 30°C, and 37°C | Homo sapiens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | - |
37 | 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 42 | - |
5 min, 55% loss of activity | Homo sapiens |
6.5.1.4 | 50 | - |
5 min, 95% loss of activity | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.4 | 8 | 9 | - |
Homo sapiens |