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Literature summary extracted from

  • Thorbjarnardottir, S.H.; Jonsson, Z.O.; Andresson, O.S.; Kristjansson, J.K.; Eggertsson, G.; Palsdottir, A.
    Cloning sequence analysis of the DNA ligase-encoding gene of Rhodothermus marinus, and overproduction, purification and characterization of two thermophilic DNA ligase (1995), Gene, 161, 1-6.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 overexpression in Escherichia coli Thermus scotoductus
6.5.1.2 overexpression in Escherichia coli Rhodothermus marinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 79487
-
 x * 79487, calculation from nucleotide sequence Rhodothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Rhodothermus marinus P49421
-
-
6.5.1.2 Thermus scotoductus
-
-
-
6.5.1.2 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2
-
 Thermus scotoductus
6.5.1.2
-
 Rhodothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus scotoductus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Rhodothermus marinus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.2 ? x * 79487, calculation from nucleotide sequence Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.2 55
-
-
 Rhodothermus marinus
6.5.1.2 65
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.2 5 75 active in this range Rhodothermus marinus
6.5.1.2 15 75 active in this range Thermus scotoductus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.2 91
-
 half-life: 7 min Rhodothermus marinus
6.5.1.2 91
-
 half-life: 26 min Thermus scotoductus