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Literature summary extracted from

  • Luo, J.; Barany, F.
    Identification of essential residues in Thermus thermophilus DNA ligase (1996), Nucleic Acids Res., 24, 3079-3085.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 C412M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C412T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C412V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C415A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C415M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C415T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C415V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C428T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C433A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C433M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C433T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 C433V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120G Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120N Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 D120Y Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 G339A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 G339D Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 G339E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K118H Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K118L Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K118P1(CCC) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K118P2(CCG) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K118R Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K2942(CTC) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K294L1(CTG) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K294P Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K294Q Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 K294R Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 R337E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 R337K Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
6.5.1.2 R337Q Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus thermophilus the enzyme plays a pivotal role in DNA replication, repair and recombination ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Thermus thermophilus
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 HB8, wild-type and site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M
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Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.2 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme plays a pivotal role in DNA replication, repair and recombination Thermus thermophilus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.2 65
-
-
 Thermus thermophilus