EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | (2E)-3-ethylcrotonoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
6.4.1.4 | (2Z)-geranoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
6.4.1.4 | butanoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
6.4.1.4 | NEM | - |
Bos taurus | |
6.4.1.4 | p-hydroxymercuribenzoate | preincubation of the enzyme with ATP or ADP protects the enzyme, while 3-methylcrotonoyl-CoA provides only very slight protection | Bos taurus | |
6.4.1.4 | Phenylglyoxal | preincubation with ATP or ADP protects, preincubation with 3-methylcrotonoyl-CoA does not | Bos taurus | |
6.4.1.4 | propanoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.4 | 0.022 | - |
(2Z)-3-ethylcrotonoyl-CoA | - |
Bos taurus | |
6.4.1.4 | 0.075 | - |
3-methylcrotonoyl-CoA | - |
Bos taurus | |
6.4.1.4 | 0.082 | - |
ATP | kidney enzyme | Bos taurus | |
6.4.1.4 | 0.225 | - |
(E)-crotonoyl-CoA | - |
Bos taurus | |
6.4.1.4 | 1.8 | - |
HCO3- | kidney enzyme | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | Co2+ | divalent cation required, Co2+ activates at 34% of the efficiency relative to Mg2+ | Bos taurus | |
6.4.1.4 | K+ | activates 7fold at 50 mM. Relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
6.4.1.4 | Li+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
6.4.1.4 | Mg2+ | divalent cation required, Mg2+ is the best activator | Bos taurus | |
6.4.1.4 | Mn2+ | divalent cation required. Mn2+ activates with 85% of the efficiency relative to Mg2+ | Bos taurus | |
6.4.1.4 | Na+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
6.4.1.4 | NH4+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 61000 | - |
x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
6.4.1.4 | 73500 | - |
x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
6.4.1.4 | 835000 | - |
PAGE, gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.4 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.4.1.4 | kidney | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 3.7 | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.4 | -20°C, 4 months, less than 20% loss of activity | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.4 | ATP + (2Z)-3-ethylcrotonoyl-CoA | 12% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ADP + phosphate + 3-ethylglutaconyl-CoA | - |
? | |
6.4.1.4 | ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Bos taurus | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
6.4.1.4 | ATP + acetoacetyl-CoA + HCO3- | 12% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ? | - |
? | |
6.4.1.4 | ATP + crotonoyl-CoA + HCO3- | (E)-crotonoyl-CoA | Bos taurus | ? | - |
? | |
6.4.1.4 | ATP + crotonoyl-CoA + HCO3- | 27% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ? | - |
? | |
6.4.1.4 | UTP + 3-methylcrotonoyl-CoA + HCO3- | at 62% of the activity relative to ATP | Bos taurus | UDP + phosphate + 3-methylglutaconyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.4 | ? | x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 38 | - |
- |
Bos taurus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 23 | 50 | 23°C: 60% of maximal activity, 30°C: 75% of maximal activity, 50°C: 15% of maximal activity | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 8 | - |
in presence of Mg2+ and K+ | Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 8 | 9 | pH 6.0: no activity, 8.0: maximal activity, pH 9.0: 50% of maximal activity | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | biotin | enzyme contains biotin | Bos taurus | |
6.4.1.4 | biotin | contains 1 mol biotin per 157000 MW AB protomeric structure | Bos taurus |