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Literature summary extracted from

  • Kalousek, F.; Darigo, M.D.; Rosenberg, L.E.
    Isolation and characterization of propionyl-CoA carboxylase from normal human liver. Evidence for a protomeric tetramer of nonidentical subunits (1980), J. Biol. Chem., 255, 60-65.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.4.1.3 limited proteolysis with trypsin results in slow time-dependent deactivation of the enzyme with preferential cleavage of the smaller subunit Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.3 0.08
-
 ATP
-
 Homo sapiens
6.4.1.3 0.29
-
 propanoyl-CoA
-
 Homo sapiens
6.4.1.3 1.2
-
 butanoyl-CoA
-
 Homo sapiens
6.4.1.3 3
-
 HCO3-
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.3 K+ stimulates, Km: 9.4 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.3 56000
-
 x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens
6.4.1.3 72000
-
 x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens
6.4.1.3 540000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.3 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.3 20
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.3 ATP + acetyl-CoA + HCO3- at a rate 1.5% that obtained for propionyl-CoA Homo sapiens ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.3 ATP + butanoyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + ?
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.3 ? x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.3 -50 37 stable Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.3 7.2 8.8
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.3 7.2 9.6 7.2-8.8: maximal activity, 9.6: about 50% of maximal activity Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.4.1.3 6.2 8.4 stable Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.3 biotin biotin-containing enzyme Homo sapiens
6.4.1.3 biotin 4 mol of biotin bound per mol of enzyme Homo sapiens