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Literature summary extracted from

  • Salto, R.; Lahoz, J.M.; del Mar Sola, M.; Oliver, J.; Vargas, A.M.
    Purification and characterization of pyruvate carboxylase from rat-kidney cortex (1990), Biochem. Arch., 6, 385-395.
No PubMed abstract available

General Stability

EC Number General Stability Organism
6.4.1.1 dilution results in irreversible inactivation, which can be partially avoided by addition of acetyl-CoA. Glycerol stabilizes Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.1 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 124700
-
 4 * 124700, SDS-PAGE Rattus norvegicus
6.4.1.1 501000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.1 kidney cortex Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 18.62
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Rattus norvegicus ADP + phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 tetramer 4 * 124700, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.1 7.6 8.4
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.1 7 9.3 7.0: about 65% of maximal activity, 9.3: about 35% of maximal activity Rattus norvegicus