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Literature summary extracted from

  • Gurr, J.A.; Jones, K.M.
    Purification and characterization of pyruvate carboxylase from Arthrobacter globiformis (1977), Arch. Biochem. Biophys., 179, 444-455.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetyl-CoA activates Arthrobacter globiformis
6.4.1.1 acetyl-CoA in absence of acetyl-CoA the maximal rate of oxaloacetate synthesis is 4% of that obtained in presence of saturating concentrations of acetyl-CoA Arthrobacter globiformis

General Stability

EC Number General Stability Organism
6.4.1.1 acetyl-CoA and sodium pyruvate protects against cold inactivation. L-Asp decreases inactivation, 1 M sucrose gives complete protection Arthrobacter globiformis
6.4.1.1 the presence of KCl at 10, 20, 50, and 100 mM potassium phosphate buffer-KCl buffer at 0°C leads to 85%, 44%, 17% and 0% loss of activity Arthrobacter globiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 Avidin
-
 Arthrobacter globiformis
6.4.1.1 L-Asp
-
 Arthrobacter globiformis
6.4.1.1 Na+ slight Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.08
-
 ATP
-
 Arthrobacter globiformis
6.4.1.1 0.33
-
 HCO3-
-
 Arthrobacter globiformis
6.4.1.1 1.86
-
 pyruvate
-
 Arthrobacter globiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 K+ increases activity by a maximum of 27% at 70 mM Arthrobacter globiformis
6.4.1.1 Mg2+ activates Arthrobacter globiformis
6.4.1.1 Mg2+ Km: 0.7 mM Arthrobacter globiformis
6.4.1.1 NH4+ increases activity by a maximum of 39% at 80 mM Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 130000
-
 4 * 130000, SDS-PAGE Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Arthrobacter globiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 27
-
-
 Arthrobacter globiformis

Storage Stability

EC Number Storage Stability Organism
6.4.1.1 0°C, 10 mM potassium phosphate, 10 mM KCl, 1 mM EDTA, 0.1 mM DTT, pH 6.8, potassium phosphate-KCl buffer, 50% loss of activity after 10 min Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Arthrobacter globiformis ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- ATP in form of MgATP2- Arthrobacter globiformis ADP + phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 tetramer 4 * 130000, SDS-PAGE Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.1 additional information
-
 cold inactivation Arthrobacter globiformis
6.4.1.1
-
-
 10 mM potassium phosphate, 10 mM KCl, 1 mM EDTA, 0.1 mM DTT, pH 6.8, potassium phosphate-KCl buffer, 50% loss of activity after 10 min Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.1 6.6
-
 in presence of 5 mM Mn2+ Arthrobacter globiformis
6.4.1.1 7.6
-
 in presence of 5 mM Mg2+ Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin enzyme contains biotin Arthrobacter globiformis