Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Charles, A.M.; Willer, D.W.
    Pyruvate carboxylase from Thiobacillus novellus: properties and possible function (1984), Can. J. Microbiol., 30, 532-539.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetoacetyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 acetyl-CoA best activator Starkeya novella
6.4.1.1 acetyl-CoA activation in decreasing order: acetyl-CoA, n-propanoyl-CoA, n-butanoyl-CoA, malonyl-CoA, CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 acyl-CoA derivatives activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 malonyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 n-butanoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 n-propanoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
6.4.1.1 oleoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella

General Stability

EC Number General Stability Organism
6.4.1.1 freezing and thawing over a 16 h period results in rapid loss of activity Starkeya novella

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 ADP
-
 Starkeya novella
6.4.1.1 CTP in presence of ATP Starkeya novella
6.4.1.1 GTP in presence of ATP Starkeya novella
6.4.1.1 HgCl2 addition of Cys results in restoration of significant activity Starkeya novella
6.4.1.1 Hydroxypyruvate
-
 Starkeya novella
6.4.1.1 ITP in presence of ATP Starkeya novella
6.4.1.1 Mercuric acetate addition of Cys results in restoration of significant activity Starkeya novella
6.4.1.1 oxaloacetate
-
 Starkeya novella
6.4.1.1 p-hydroxymercuribenzoate
-
 Starkeya novella
6.4.1.1 phosphate
-
 Starkeya novella
6.4.1.1 Tris
-
 Starkeya novella
6.4.1.1 UTP in presence of ATP Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.04
-
 ATP
-
 Starkeya novella
6.4.1.1 0.25
-
 pyruvate
-
 Starkeya novella
6.4.1.1 0.27
-
 HCO3-
-
 Starkeya novella

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 Co2+ divalent cation required, activates with 58% of the efficiency relative to Mg2+ Starkeya novella
6.4.1.1 Cs+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
6.4.1.1 K+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
6.4.1.1 Mg2+ divalent cation required, Mg2+ is most effective, Km: 0.44 mM Starkeya novella
6.4.1.1 Mn2+ divalent cation required, activates with 66% of the efficiency relative to Mg2+ Starkeya novella
6.4.1.1 NH4+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
6.4.1.1 Rb+ monovalent cation required, Rb+ is most effective Starkeya novella

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 1.03
-
-
 Starkeya novella

Storage Stability

EC Number Storage Stability Organism
6.4.1.1 -20°C, pH 7.8, 2 weeks, 40% loss of activity Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Starkeya novella ADP + phosphate + oxaloacetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.1 25 35
-
 Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.1 7.6
-
-
 Starkeya novella